Artocarpus integrifolia Lectin (AIA) - Pure

Product Description
Jacalin lectin AIA is isolated from jackfruit seeds (Artocarpus integrifolia) and purified by affinity chromatography. AIA is a homotetrameric two-chain structure consisting of four identical protomers and a molecular weight of 66,000, with an isoelectric point of pH 7.8. Jacalin is considered a galactose-specific lectin that has an eluting concentration of 0.2M of galactose or melibiose. A post-translational proteolytic modification of Jacalin gives the lectin a novel carbohydrate-binding site involving the N terminus of the a-chain. The protein’s structure explains it’s carbohydrate-binding specificity of T-antigen disaccharide GalBeta1,3GalNAc. Potent inhibitors of jacalin include D-galactose, Beta-Met-Gal and 2-deoxy-α-D-galactose. The lectin has specificity for blood group O (+SA) and T.

This product comes in a lyophilized powder form and is stable for more than five years from production date when stored below -20 C.

Applications:
Isolation of IgA from human serum
Isolation of human plasma glycoproteins
Histochemistry
AIDS research

References

• Bourne, Y., et al. Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannose. J. Biochem. (2002) 364:173-180.
  
• Liener I. E., Sharon N., Goldstein I. J. The Lectins - Properties, Functions and Applications in Biology and Medicine. (1986).
  
• Sankaranarayanan, R., et al. A novel mode of carbohydrate recognition in jacalin, a Moraceae plant lectin with a b-prism fold. Nature Structural Biology. (1996) 3:596-603.