Artocarpus integrifolia Lectin (AIA) - Separopore® 4B

Applications:

Jacalin, an α D-galactose-specific lectin from jackfruit (Artocarpus integrifolia), specifically binds human IgA1 (called secretory IgA or serum IgA) and does not bind other immunoglobulins such as IgG, human IgA2, or IgA from other species. Jacalin coupled Separopore is used for the purification of glycoproteins containing α-D-galactose, specifically those with O-glycosidically linked oligosaccharide side chains. . Jacalin-Separopore 4B-CL is used for purification of glycoproteins containing α-D-galactose and is useful for purification of human serum (orsecretory) IgA1. IgA can be separated from human IgG and IgM in human serum or colostrums using immobilized Jacalin. This support is also useful for removing contaminating IgA from IgG samples. Additionally, Jacalin binds IgD. Jacalin can also be used to separate IgA1 subclass from IgA2.

Technical Specifications

• Ligand Source: Artocarpus integrifolia
• Matrix: Separopore 4B-CL (crosslinked agarose, 4%)
• Particle size range: 52 - 165 µm
• Matrix activation: Cyanogen bromide
• Matrix attachment: Amino
• Ligand density: 4.5 mg Jacalin / ml drained gel matrix
• Binding Capacity: 1 - 3 mg human IgA1 /mL drained gel
• Inhibiting/Eluting Sugar: Galactose or Melibiose
• Carbohydrate for Elution: .2M Galactose or Melibiose
• Blood Group Specificity: 0 (+SA), T antigen
• Form: 50:50 Liquid Suspension, PBS (1x) pH 7.4 w/ 0.01% Thimerosal
• pH stability: 4 - 9
• Storage: 2-8 C, DO NOT FREEZE
• Application note: N/A

References:

• Serological reactivity of different antigenic preparations of Leishmania (Leishmania) amazonensis and the Leishmania braziliensis complex. Rev Soc Bras Med Trop. (2008) 41: 135-41.
• A comparison of the binding of secretory component to immunoglobulin A (IgA) in human colostral S-IgA1 and S-IgA2. Immunology. (2007) 120: 273-80.
• Disordered balance of IgA subclass production in the tonsils of some IgA nephropathy patients. J Nephrol. (2005) 18: 575-81.
• Compositional similarity between immunoglobulins binding to asialo-, agalacto-IgA1-Sepharose and those deposited in glomeruli in IgA nephropathy. J Nephrol. (2004) 17: 679-86.
• Hypersialylated macromolecular serum immunoglobulin A1 in type 2 diabetes mellitus. Clin Biochem. (2001) 34: 35-41.
• Characterization of the rabbit homolog of human MUC1 glycoprotein isolated from bladder by affinity chromatography on immobilized jacalin. Glycobiology. (2000) 10: 659-67.
• Increased sialylation of polymeric immunoglobulin A1: mechanism of selective glomerular deposition in immunoglobulin A nephropathy? J Lab Clin Med. (1999) 133: 152-60.
• Serologic study of immunoglobulin A-fibronectin aggregates in immunoglobulin A nephropathy. Am J Kidney Dis. (1996) 27: 622-30.
• Increased binding of polymeric lambda-IgA to cultured human mesangial cells in IgA nephropathy. Kidney Int. (1996) 49: 839-45.
• Identification and characterization of human serum alpha2-HS glycoprotein as a jacalin-bound protein. Biochim Biophys Acta. (1995) 1249: 58-64.
• Mucin-like glycoproteins in the equine embryonic capsule. Mol Reprod Dev. (1993) 34: 255-65.
• Poly-N-acetyllactosaminyl O-glycans attached to leukosialin. The presence of sialyl Le(x) structures in O-glycans. J Biol Chem. (1992) 267: 24379-86.
• Porcine oocyte zona pellucida M(r) 55,000 glycoproteins: identification of O-glycosylated domains. Mol Reprod Dev. (1992) 33: 182-8.
• Isolation of glycopeptides containing O-linked oligosaccharides by lectin affinity chromatography on jacalin-agarose. Anal Biochem. (1990) 191: 262-7.
• Sulfation of tyrosine residues in coagulation factor V. Blood. (1990) 76: 946-52.
• Lectin affinity chromatography of proteins bearing O-linked oligosaccharides: application of jacalin-agarose. Anal Biochem. (1990) 188: 271-7.
• A new simplified procedure for C1 inhibitor purification. A novel use for jacalin-agarose. J Immunol Methods. (1989) 120: 37-43.
• Separation of human IgA1 and IgA2 using jacalin-agarose chromatography. J Immunol Methods. (1987) 99: 101-6.

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Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.