Calcium Colorimetric Assay Kit (Chlorophosphonazo-III Method)

Product description: Calcium is the most abundant mineral, essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% is present as calcium hydroxyapatite in bones and less than 1 % is present in the extra-osseous ICS (intracellular space) or ECS (extracellular space), responsible for a myriad of important physiological effects. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Calcium ions affect the contractility of the heart and the skeletal musculature and are essential for the function of the nervous system. In addition, calcium ions play an important role in blood clotting and bone mineralization. Abnormal calcium levels have been tied to neurological, endocrine, cardiovascular, digestive, metabolic, and pulmonary diseases, as well as cancer. In plasma, calcium is bound to a considerable extent to proteins (approx. 40%), 10% is in the form of incorganic complexes and 50% is present as free (ionized) calcium. Clacium homeostasis is regulated by the parathyroid hormone (PTH), calcitriol (CT) and calcitonin. Low levels of calcium are found in hypoparathyroidism, pseudohypoparathyroidism, vitamin D deficiency, malnutrition and intestinal malabsorbtion.

Product Type: Kit

Specificity: Specific to Ca2+.

Crossreactivity: All

Kit Contains: 1 x 50 ml R-1 Chelate color (ready to use) 1 x 1.0 ml STD Calcium (Ca) standard 10mg/dL (ready to use)

Range: 0.2 - 20mg/dL

Sample Type: Cell Lysate, Plasma, Saliva, Serum, Tissue Supernatant, Urine

Detection Type: Colorimetric

Other data: This MC-Reagent Calcium Assay Kit is a direct colorimetric assay based on the Chlorophosphonazo-III (CPZ-III) method without deproteinization of the sample. The calcium determination is based on the reaction of calcium with Chlorophosphonazo-III (as chelator) at neutral pH, which yields a blue colored complex. The intensity of the color formed is proportional to the calcium concentration in the sample. Absorbance of the Ca2+-CPZ-III complex is measured at 690nm. Wavelength range of sensitivity: 680 ~ 700nm. Features of this Assay: Quick & Easy to use • Species independent • Highly sensitive, stable and suitable for high-throughput testing • No toxic substances • For cell lysates, serum, plasma and wide variety of biological samples

Short Term Storage: +4°C

Long Term Storage: +4°C

Shipping: BLUE ICE

Use & Stability 12 months after the day of manufacturing. See expiry date on ELISA Kit box.

Handling Advice Do not freeze.

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