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96 wells

Ref. AG-45B-0002-KI01
ADIPOGEN LIFE SCIENCES

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Neuf 540.00 486.00 H.T.

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Détails Produit

Product description: Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1beta (IL-1beta) and interleukin-18 (IL-18) and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3/NALP3 responds to a variety of agonists including crystals. This Caspase-1 (mouse) ELISA Kit can be used to measure caspase-1 (mouse) in cell culture supernatants, serum and plasma. It is a quantitative detection method, alternative to Western blotting to measure caspase-1 secretion.

Alternate Names/Synonyms: Interleukin-1beta Convertase; IL-1BC; Interleukin-1beta-converting Enzyme; ICE

Product Type: Kit

Specificity: Detects mouse caspase-1 (proform as well as p10 and p20 domain). Does not detect human caspase-1.

Crossreactivity: Mouse

Sensitivity: 33pg/ml

Range: 0.15ng/ml to 10ng/ml

Sample Type: Cell Culture Supernatant, Plasma, Serum

Assay Type: Sandwich

Detection Type: Colorimetric

Short Term Storage: +4°C

Long Term Storage: +4°C

Shipping: BLUE ICE

Use & Stability 12 months after the day of manufacturing. See expiry date on ELISA Kit box.

Handling Advice After standard reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Plate and reagents should reach room temperature before use.

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Manuel Kit