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1 Set

Ref. AG-46B-0003-KI01
ADIPOGEN LIFE SCIENCES

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Neuf 560.00 504.00 H.T.

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Détails Produit

Product description: Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1beta (IL-1beta) and interleukin-18 (IL-18) and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3/NALP3 responds to a variety of agonists including crystals. The caspase-1 (mouse) Matched Pair Detection Set contains all reagents for the development of a sandwich ELISA to measure caspase-1 (mouse) in cell culture supernatants. This Matched Pair Detection Set is a quantitative detection method, alternative to Western blotting to measure caspase-1 secretion.

Alternate Names/Synonyms: Interleukin-1beta Convertase; IL-1BC; Interleukin-1beta-converting Enzyme; ICE

Product Type: Set

Specificity: Detects mouse Caspase-1 (p10 and p20 domain). Does not detect human Caspase-1.

Crossreactivity: Mouse

Kit Contains: 1 vial standard protein (1µg) (lyophilized) [STD] 1 vial coating antibody (120µg) [COAT] 1 vial detection antibody (60µg) [DET] 1 vial streptavidin-HRP (10µg) (lyophilized) [STREP]

Sensitivity: 50pg/ml

Range: 0.15ng/ml to 10ng/ml.

Sample Type: Cell Culture Supernatant

Assay Type: Sandwich

Detection Type: Colorimetric

Short Term Storage: -20°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability Stable for at least 1 year after receipt when stored at -20°C.

Handling Advice Avoid freeze/thaw cycles.

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Manuel Kit