CD152 [CTLA-4] (mouse):Fc (mouse) (rec.)

Product Description: CD152 and CD28, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD152 and CD28 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD152 and CD28 are composed of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. CD152 and CD28 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. CD152 was originally identified as a gene that was specifically expressed by cytotoxic T lymphocytes. However, CD152 transcripts have since been found in both Th1 and Th2, and CD4+ and CD8+ T cell clones. Whereas, CD28 expression is constitutive on the surfaces of 95% of CD4+ T cells and 50% of CD8+ T cells and is down regulated upon T cell activation, CD152 expression is upregulated rapidly following T cell activation and peaks approximately 24 hours following activation. Although both CD152 and CD28 can bind to the same ligands, CD152 binds to B71 and B72 with 20-100-fold higher affinity than CD28.

Alternate Names/Synonyms: CTLA-4

Product type: Protein

Source / Host: NS1 cells

Sequence: The extracellular domain of mouse CD152 [CTLA-4] (aa 38-160) is fused to the N-terminus of the Fc region of mouse IgG2a.

Species Crossreactivity: Mouse

Biological Activity: Binds both CD80 (B7-1) and CD86 (B7-2) with high affinity and inhibits CD28 signaling competitively. Kills the target cell completely.

TAGs: Fc

Purity: >98% (SDS-PAGE)

Endotoxin: <0.06EU/µg protein (LAL test; Lonza).

Formulation: Lyophilized from 0.2µm-filtered solution in PBS.

Handling Advice: Avoid freeze/thaw cycles.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

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