CD40L (human) (multimeric) (rec.) (Certified Serum Grade)

Product description: Recombinant protein. Human CD40L (aa 116-261) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag. Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) (ED50: <1ng/ml). B cell expansion online protocol available. Source: CHO cells. Produced using certified serum/medium.*. Liquid. Contains PBS. MultimericCD40L™ is a high-activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to [CD40L+enhancer] combinations. MultimericCD40L™ has been shown to suppress alum-induced IL-1beta release and caspase-1 activation in a dose-, CD40- and time-dependent manner, without affecting BMDM viability. It also effectively suppressed the inflammasome function triggered by NLRP3 activators. The secretion of caspase-1 independent inflammatory mediators has been shown to be unaltered or even enhanced. MultimericCD40L™ has been shown to be a potent tool for B cell expansion. It also has big potential as a growth factor for tumor-infiltrating lymphocytes (TILs), which have been shown to be important for T cell therapy.

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