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The Experts for High Quality Fusion Proteins

 

 

Since many years Chimerigen Laboratories, LLC (Chimerigen) develops, manufactures and markets high quality and leading edge proteins for biomedical and immunology research. One of Chimerigen's specialty is the production of unique immunoglobulin based chimeric fusion proteins using advanced cellular and molecular biological techniques. These reagents are used successfully in basic and applied research. AdipoGen has now become a trusted and reliable marketing and sales partner for Chimerigen's product panel. Because of the high performance characteristics and quality the Chimerigen fusion proteins are widely recognized reagents and are cited in many scientific publications.

 

Non-lytic Ig-based Chimeric Fusion Cytokines with Long Circulating Half-life

The potential clinical application of cytokines to modulate immune responses is very high. Unfortunately, most cytokines have short circulating half-lives. Therefore, to facilitate the study of cytokine effects in vivo, a variety of non-lytic immunoglobulin-based chimeric cytokine fusion proteins have been created, in which a cytokine sequence had been genetically fused to the hinge, CH2 and CH3 regions of an immunoglobulin. 


These non-lytic fusion proteins possess both the biological functions of the cytokine moiety and a prolonged circulating half-life determined by the Fc domain. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC). These fusion molecules also have the promise of being minimally to negligibly immunogenic since they are made entirely from elements derived from the species to be treated.

Structure of Fusion Proteins     
Figure:
General structure of mouse non-lytic fusion proteins.

Literature:
Localization of the binding site for the human high-affinity Fc receptor on IgG: A.R. Duncan, et al.; Nature 332, 563 (1988)
The binding site for Clq on IgG: A.R. Duncan & G. Winter; Nature 332, 738 (1988)
Administration of noncytolytic IL-10/Fc in murine models of lipopolysaccharide-induced septic shock and allogeneic islet transplantation: X.X. Zheng, et al.; J. Immunol. 154, 5590 (1995)

Latest Insights: IL-35 & its Involvment in Type 1 Diabetes (T1D)
 
Singh, et al. 2015, using Chimerigen's IL-35 (mouse):Fc (human) (rec.) (CHI-MF-11135) as a key reagent, recently showed that circulating IL-35 levels were decreased in human Type 1 Diabetes (T1D) patients compared to healthy controls. The findings of this study suggest that insufficient IL-35 levels play a pivotal role in the development of T1D and that treatment with IL-35 should be investigated in treatment of T1D and other autoimmune diseases.
 
LIT: Interleukin-35 administration counteracts established murine type 1 diabetes – possible involvement of regulatory T cells: K. Singh, et al.; Sci. Rep. 5, ID12633 (2015) - Link to Publication
 
PID Product Name         Size Source Purity Endotoxin
 CHI-MF-11135  IL-35 (mouse):Fc (human) (rec.) 5 µg  CHO cells  ≥98%  <0.06EU/μg
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Tim-3 (mouse):Fc (human) (rec.) Tim-3 (mouse):Fc (human) (rec.) 100 µg Ref. CHI-MF-111T3-C100 Marque CHIMERIGEN 430.00 H.T.
 
Tim-4 (human):Fc (human) (rec.) Tim-4 (human):Fc (human) (rec.) 100 µg Ref. CHI-HF-210T4-C100 Marque CHIMERIGEN 430.00 H.T.
 
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Tim-4 (human):Fc (mouse) (rec.) Tim-4 (human):Fc (mouse) (rec.) 100 µg Ref. CHI-HF-211T4-C100 Marque CHIMERIGEN 430.00 H.T.
 
Tim-4 (mouse):Fc (mouse) (rec.) Tim-4 (mouse):Fc (mouse) (rec.) 100 µg Ref. CHI-MF-110T4-C100 Marque CHIMERIGEN 430.00 H.T.
 
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Tim-4 (mouse):Fc (human) (rec.) Tim-4 (mouse):Fc (human) (rec.) 100 µg Ref. CHI-MF-111T4-C100 Marque CHIMERIGEN 410.00 H.T.
 
TNF-alpha (human) (rec.) (His) TNF-alpha (human) (rec.) (His) 10 µg Ref. CHI-HR-200TNF-C010 Marque CHIMERIGEN 100.00 H.T.
 
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TNF-alpha (human) (rec.) (His) TNF-alpha (human) (rec.) (His) 50 µg Ref. CHI-HR-200TNF-C050 Marque CHIMERIGEN 320.00 H.T.
 
VSIG4 (human):Fc (mouse) (rec.) VSIG4 (human):Fc (mouse) (rec.) 100 µg Ref. CHI-HF-211VSIG4-C100 Marque CHIMERIGEN 350.00 H.T.
 
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Fc (human) IgG1 Control (rec.) Fc (human) IgG1 Control (rec.) 100 µg Ref. CHI-HF-210IG1-C100 Marque CHIMERIGEN 310.00 H.T.
 
Fc (human) IgG1 Control (rec.) (non-lytic) Fc (human) IgG1 Control (rec.) (non-lytic) 100 µg Ref. CHI-HF-220IG1-C100 Marque CHIMERIGEN 310.00 H.T.
 
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Fc (human) IgG4 Control (rec.) Fc (human) IgG4 Control (rec.) 100 µg Ref. CHI-HF-210IG4-C100 Marque CHIMERIGEN 310.00 H.T.
 
Fc (mouse) IgG2a Control (rec.) Fc (mouse) IgG2a Control (rec.) 100 µg Ref. CHI-MF-110IG2A-C100 Marque CHIMERIGEN 310.00 H.T.
 
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Fc (mouse) IgG2a Control (rec.) (non-lytic) Fc (mouse) IgG2a Control (rec.) (non-lytic) 100 µg Ref. CHI-MF-120IG2A-C100 Marque CHIMERIGEN 310.00 H.T.
 
IL-18 (human) (rec.) IL-18 (human) (rec.) 10 µg Ref. CHI-HR-20618-C010 Marque CHIMERIGEN 135.00 H.T.
 
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IL-18 (human) (rec.) IL-18 (human) (rec.) 50 µg Ref. CHI-HR-20618-C050 Marque CHIMERIGEN 390.00 H.T.
 
GPX4 (human) (rec.) (His) GPX4 (human) (rec.) (His) 50 µg Ref. CHI-AG-40A-0159-C050 Marque CHIMERIGEN 640.00 H.T.
 
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CD279 [PD-1] (mouse):Fc (human) (rec.) CD279 [PD-1] (mouse):Fc (human) (rec.) 100 µg Ref. CHI-MF-111PD1-C100 Marque CHIMERIGEN 350.00 H.T.
 
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CD36 (human):Fc (human) (rec.) CD36 (human):Fc (human) (rec.) 100 µg Ref. CHI-HF-210CD36-C100 Marque CHIMERIGEN 430.00 H.T.
 
Thrombospondin-1 (human):Fc (human) (rec.) Thrombospondin-1 (human):Fc (human) (rec.) 50 µg Ref. CHI-HF-210TSP1-C050 Marque CHIMERIGEN 320.00 H.T.
 
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TNF-R1 (mouse):Fc (human) (rec.) TNF-R1 (mouse):Fc (human) (rec.) 50 µg Ref. CHI-MF-111TNFR1-C050 Marque CHIMERIGEN 320.00 H.T.
 
TNF-R1 (mouse):Fc (mouse) (rec.) TNF-R1 (mouse):Fc (mouse) (rec.) 50 µg Ref. CHI-MF-110TNFR1-C050 Marque CHIMERIGEN 320.00 H.T.
 
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BTNL2 (human):Fc (human) (rec.) BTNL2 (human):Fc (human) (rec.) 50 µg Ref. CHI-HF-210BTNL2-C050 Marque CHIMERIGEN 400.00 H.T.
 
BTNL2 (human):Fc (human) (rec.) BTNL2 (human):Fc (human) (rec.) 100 µg Ref. CHI-HF-210BTNL2-C100 Marque CHIMERIGEN 600.00 H.T.
 
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IL-2 (human) (rec.) (untagged) IL-2 (human) (rec.) (untagged) 10 µg Ref. CHI-HR-20602-C010 Marque CHIMERIGEN 135.00 H.T.
 
IL-2 (human) (rec.) (untagged) IL-2 (human) (rec.) (untagged) 50 µg Ref. CHI-HR-20602-C050 Marque CHIMERIGEN 350.00 H.T.
 
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IL-4 (human) (rec.) (untagged) IL-4 (human) (rec.) (untagged) 10 µg Ref. CHI-HR-20604-C010 Marque CHIMERIGEN 220.00 H.T.
 
IL-4 (human) (rec.) (untagged) IL-4 (human) (rec.) (untagged) 50 µg Ref. CHI-HR-20604-C050 Marque CHIMERIGEN 640.00 H.T.
 
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IL-10 (human) (rec.) (untagged) IL-10 (human) (rec.) (untagged) 10 µg Ref. CHI-HR-20610-C010 Marque CHIMERIGEN 220.00 H.T.
 
IL-10 (human) (rec.) (untagged) IL-10 (human) (rec.) (untagged) 50 µg Ref. CHI-HR-20610-C050 Marque CHIMERIGEN 640.00 H.T.
 
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IL-21 (human) (rec.) (untagged) IL-21 (human) (rec.) (untagged) 10 µg Ref. CHI-HR-20621-C010 Marque CHIMERIGEN 220.00 H.T.
 
IL-21 (human) (rec.) (untagged) IL-21 (human) (rec.) (untagged) 50 µg Ref. CHI-HR-20621-C050 Marque CHIMERIGEN 640.00 H.T.
 
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beta-Amyloid 40 (human):Fc (human) (rec.) beta-Amyloid 40 (human):Fc (human) (rec.) 100 µg Ref. CHI-HF-210BA40-C100 Marque CHIMERIGEN 430.00 H.T.
 
beta-Amyloid 42 (human):Fc (human) (rec.) beta-Amyloid 42 (human):Fc (human) (rec.) 100 µg Ref. CHI-HF-210BA42-C100 Marque CHIMERIGEN 430.00 H.T.
 
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CD28 (human) (rec.) (His) CD28 (human) (rec.) (His) 100 µg Ref. CHI-HF-201CD28-C100 Marque CHIMERIGEN 320.00 H.T.
 
CXCL13 [BCA1] (human):Fc (mouse) (rec.) CXCL13 [BCA1] (human):Fc (mouse) (rec.) 50 µg Ref. CHI-HF-211BCA1-C050 Marque CHIMERIGEN 320.00 H.T.
 
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FLT3 Ligand (human):Fc (mouse) (rec.) FLT3 Ligand (human):Fc (mouse) (rec.) 50 µg Ref. CHI-HF-211FLT3L-C050 Marque CHIMERIGEN 370.00 H.T.
 
GDF15 (human):Fc (human) (rec.) GDF15 (human):Fc (human) (rec.) 100 µg Ref. CHI-HF-210GDF15-C100 Marque CHIMERIGEN 430.00 H.T.
 
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GDF15 (human):Fc (mouse) (rec.) GDF15 (human):Fc (mouse) (rec.) 100 µg Ref. CHI-HF-211GDF15-C100 Marque CHIMERIGEN 430.00 H.T.
 
GDF15 (mouse):Fc (mouse) (rec.) GDF15 (mouse):Fc (mouse) (rec.) 100 µg Ref. CHI-MF-110GDF15-C100 Marque CHIMERIGEN 430.00 H.T.
 
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GDF15 (mouse):Fc (human) (rec.) GDF15 (mouse):Fc (human) (rec.) 100 µg Ref. CHI-MF-111GDF15-C100 Marque CHIMERIGEN 430.00 H.T.
 
GFRAL (human):Fc (human) (rec.) GFRAL (human):Fc (human) (rec.) 100 µg Ref. CHI-HF-210GFRAL-C100 Marque CHIMERIGEN 430.00 H.T.
 
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GFRAL (mouse):Fc (mouse) (rec.) GFRAL (mouse):Fc (mouse) (rec.) 100 µg Ref. CHI-MF-110GFRAL-C100 Marque CHIMERIGEN 430.00 H.T.
 
IL-22 (human):Fc (mouse) (rec.) IL-22 (human):Fc (mouse) (rec.) 25 µg Ref. CHI-HF-21122-C025 Marque CHIMERIGEN 255.00 H.T.
 
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IL-22 (human):Fc (mouse) (rec.) IL-22 (human):Fc (mouse) (rec.) 50 µg Ref. CHI-HF-21122-C050 Marque CHIMERIGEN 340.00 H.T.
 
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