Cicer arietinum Lectin (CAL/CPA) - Pure

Product Description
Lectin/agglutinin CAL/CPA is isolated from chickpeas (Cicer arietinum). CAL is identified as a homodimer composed of two subunits confirmed by SDS-PAGE. CAL has the molecular weight of 43,000 Da determined by gel filtration with use of HPLC. The lectin is a glycoprotein that contains 4.5% neutral sugars and is basic in nature (pI 9.0). It’s haemagglutination activity is inhibited by fetuin and desialated fetuin with a carbohydrate specificity for fetuin and p-Nitrophenyl N-acetyl-α-D-galactoside. CAL possess complex-sugar specificity for N-glycans and N-acetyl-D-galactosamine, with an elution concentration of 0.2M of acetyl-D-galactosamine. Although neither human or rabbit eyrthrocytes are agglutinated by this lectin, pronase-treated rabbit and human erythrocytes have been successfully agglutinated.

This product comes in a lyophilized powder form and is stored at -20 C.

References

• Agarwal, S. and Agarwal, S. S. Ind. J. Med. Res. (1990) 92:38-42.
• Katre, U., et al. Crystallization and preliminary X-ray characterization of a lectin from Cicer arietinum (chickpea). Acta Crystallogr Sect F Struct Biol Cryst commun. (2004) 61:141-143
• Kolberg, J., et al. Properties of a lectin purified from the seeds of Cicer arietinum. Hoppe-Seyler’s Z. Physiol. Chem. (1983) 364:655-664.
• Wine, R. N. and Chapin, R. E. J. Andrology. (1997) 18:71-79.