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AVANTI POLAR LIPIDS , CYGNUS , LARODAN , ADIPOGEN , MYBIOSOURCE , SERVA NORDMARK, BIOWORLD, EDGEBIO , PANREAC APPLICHEM
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Collagenase AF-1 GMP Grade Nr: N0003554
2000 U (PZ)
photos non contractuelles
Détails Produit
World's first animal free GMP Grade Collagenase
Collagenase AF-1 GMP Grade is derived from C. histolyticum using a production process completely free of any animal based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This highly purified collagenase, often in combination with Neutral Protease AF GMP Grade (Cat. No. N0002936), is suitable for the dissociation of sensitive cells from several tissues, such as pancreas, liver and dental pulp. It has been shown to be highly effective for the isolation of islets of Langerhans from human pancreas intended for transplantation.
Application:
Collagenase AF-1 GMP can be used for several cell isolations, but is commonly used for human islet isolation.
Specifications:
PZ activity (Wünsch) ≥ 3.00 U/mg*.
Endotoxin ≤ 10.0 EU**/mg (Ph. Eur.)
Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia.
Endproduct is 0.22 µm filtered.
Manufactured according to EU GMP guidelines.
(Clostridiopeptidase A, Collagenase A)
EC 3.4.24.3 • Mr Mr 70 000 - 120 000 (collagenase) • CAS [9001-12-1]
Order Information
| Productname | Cat.No | Size | EUR |
| Collagenase AF-1 GMP Grade |
Nordmark : N0003554 Serva : 1745701 |
≥ 2,000 U* | Inquire |
Storage Temperature: +2 °C to +8 °C
This article will be delivered cooled on blue ice.
References and Definitions
*Unit definitions: Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
**Endotoxin: Ph. Eur. [1 Endotoxin Unit is equal to 1 International Unit of a WHO appoved reference standard endotoxin (RSE)].
References:
- Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
- Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler´s Z. Physiol. Chem. 333, 149-51
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