Collagenase NB 5 Sterile Grade Nr: N0002778

Nordmark Collagenase NB - first choice for high yield and viable cells

Collagenase NB 5 Sterile Grade is applicable to tissue dissociation such as isolation of human and rodent hepatocytes, chondrocytes, neuronal and endothelial cells, fibroblasts, cells from adipose tissue (e.g. ADSCs), stem cells and rodent osteoblasts.
See Collagenase NB 6 GMP Grade (Cat. no. N0002779) for clinical applications like tissue engineering and transplantation.

More about Applications

Contains class I and class II collagenase (1) and a balanced ratio of proteolytic side activities.
PZ activity (Wünsch): ≥ 0.10 U/mg
Microbiological examination: Sterility, absence of Clostridia
Sterile according to Ph. Eur.
(Clostridiopeptidase A)
EC 3.4.24.3 • M_r ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]

Storage Temperature: +2 °C to +8 °C
 

For initial testing and protocol optimization, Collagenase NB 4 Standard Grade (Cat. No. S1745401) can be used. If the application has to meet GMP requirements, Nordmark also offers Collagenase NB 6 GMP Grade which has comparable enzymatic properties to Collagenase NB 5 Sterile Grade and Collagenase NB 4 Standard Grade (Cat. No. S1745401).

Unit definition: 1 U according to Wünsch (2) catalyzes the hydrolysis of
1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.

References:
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51

Product Information
Material Safety Datasheet
Technical Notes
CoO (Certificate of Origin)
Certificate of Analysis (Lotsearch)