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Collagenase NB 8 Broad Range Nr: S1745601
250 mg
photos non contractuelles
Détails Produit
Nordmark Collagenase NB - first choice for high yield and viable cells
Suitable for a broad range of different applications in tissue dissociation such as isolation of rodent pancreatic islets of Langerhans, human cardiomyocytes, tumor cells, muscle cells, osteoblasts and porcine hepatocytes. Increased collagenase activity compared to Collagenase NB 4 Standard Grade (Cat. no. S1745401) and Collagenase NB 4G Proved Grade (Cat. no. S1746501).
Specifications:
Contains class I and class II collagenase (1) as well as reduced levels of clostripain, trypsin-like activities and neutral protease.
Chromatographically purified.
PZ activity (Wünsch): ≥ 0.90 U/mg
Endotoxin: ≤ 100 EU/mg (Ph. Eur.)
(Clostridiopeptidase A)
EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]
Order Information
| Product name | Cat.No | Size | EUR |
| Collagenase NB 8 Broad Range |
Nordmark : S1745601 Serva : 1745601 |
250 mg |
Inquire+ |
|
Collagenase NB 8 Broad Range |
Nordmark : S1745602 Serva : 1745602 |
1 g | Inquire |
Storage Temperature: +2 °C to +8 °C
This article will be delivered cooled on blue ice.
Related Products
If you are dealing with poor cell yields, collagenases with higher side activities like Collagenase NB 4 Standard Grade (Cat. No. S1745401) and Collagenase NB 4G Proved Grade (Cat. Co. S1746502).
References and Definitions
Unit definition:
Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of
1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
References:
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51.
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