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AVANTI POLAR LIPIDS , CYGNUS , LARODAN , ADIPOGEN , MYBIOSOURCE , SERVA NORDMARK, BIOWORLD, EDGEBIO , PANREAC APPLICHEM
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Product Description
Pure lectin DSA/DSL is isolated from jimson weed (Datura stramonium) and was purified by affinity chromatography. DSL is a glycoprotein that contains 40% carbohydrate. DSL binds well to N-acetylglucosamine, oligomers and branched pentasaccharide, including two N-acetylglucosamine disaccharides linked to mannose (Beta-1,6) and (Beta-1,2) which is known to be the most potent inhibitor of agglutination. DSL prefers binding to (GlcNAc)2-4 and elutes 0.2M concentration of chitin hydrolysate. This agglutinin has blood group A, B, and O specificity.
DSL binds well in the acidic pH range but its affinity decreases above pH 8.0. DSL contains two chains of 40 and 46 kDa joined by disulfide bonds, and has a molecular weight of 86 kDa.
DSL product comes in a lyophilized powder form and is stable for 12 months from production date when stored at -20 C. Reconstitute with sterile buffer. Reconstituted product is stable for 6 months at -20 C. No metal ions are required for binding.
Applications:
Glycobiology
Mitogenic stimulation
Suitable for use in ELISA
References
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