DEAE Separopore ® (Agarose) 6B-CL

Applications: DEAE-Separopore 6B-CL is a weak anion exchanger with excellent flow capacities and a high capacity for binding and resolution of proteins of all pI values. bio-World's DEAE-Separopore 6B-CL was prepared by covalent attachment of diethylaminoethyl (DEAE) to Separopore 6B-CL. DEAE remains charged and maintains high capacity over a wide working range of pH 3 - 9. It is an excellent matrix for both analytical and preparative fractionation of proteins.

Technical specifications:

• Ligand: Diethylaminoethyl (DEAE)
• Matrix: Separopore 6B-CL (crosslinked agarose beads, 6%)
• Particle size range: 52 - 165 μm
• Molecular weight range: 6 x 10⁴ - 2 x 10⁷
• Ligand coupling method: CNBr activation
• Total ionic capacity: 0.13 - 0.17 mmol (Cl^-) / ml drained gel
• Binding capacity: 50 - 170 mg protein / ml drained gel
• Flow specifications: 70 - 140 cm / h
• pH stability: 3 - 9
• Storage: 2 - 8 C
• Supplied as pre-swollen gel in 20% ethanol

References:

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• A novel Ca2+-dependent phospholipase D from Streptomyces tendae, possessing only hydrolytic activity. Arch Pharm Res. (2009) 32: 1461-7.
• A glycoprotein extracted from golden oyster mushroom Pleurotus citrinopileatus exhibiting growth inhibitory effect against U937 leukemia cells. J Agric Food Chem. (2009) 57: 6706-11.
• An effective method for hemoglobin E detection: DEAE sepharose microcolumn. Lab Hematol. (2009) 15: 10-2.
• Antioxidative activity of polysaccharide fractions isolated from Lycium barbarum Linnaeus. Int J Biol Macromol. (2009) 45: 146-51.
• Structural characterization and anti-fatigue activity of polysaccharides from the roots of Morinda officinalis. Int J Biol Macromol. (2009) 44: 257-61.
• Purification and characterization of a cathepsin L-like enzyme from the body wall of the sea cucumber Stichopus japonicus. Biosci Biotechnol Biochem. (2008) 72: 1430-7.
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• Purification and characterization of arginine kinase from locust. Protein Pept Lett. (2006) 13: 405-10.
• Structural investigation of a fucoidan containing a fucose-free core from the brown seaweed, Hizikia fusiforme. Carbohydr Res. (2006) 341: 1135-46.
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• Purification and characterization of a fructosyltransferase from onion bulbs and its key role in the synthesis of fructo-oligosaccharides in vivo. New Phytol. (2005) 165: 513-24.
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• Purification and characterization of a novel prolyl aminopeptidase from Maitake (Grifola frondosa). Biosci Biotechnol Biochem. (2004) 68: 1395-7.
• Cloning and expression of the glucose-1-phosphate thymidylyltransferase gene (gerD) from Streptomyces sp. GERI-155. Mol Cells. (2004) 17: 274-80.
• Expression, localization, and correlation of N-myristoyltransferase and its inhibitor in bovine eye. Invest Ophthalmol Vis Sci. (2004) 45: 1674-9.
• Human salivary aggregation in Streptococcus intermedius type g strains: relationship with IgA. FEMS Immunol Med Microbiol. (2004) 41: 101-7.
• Partial purification of an enzyme hydrolyzing indole-3-acetamide from rice cells. J Plant Res. (2004) 117: 191-8.
• NADH-ubiquinone oxidoreductase activity in the kinetoplasts of the plant trypanosomatid Phytomonas serpens. Parasitol Res. (2004) 92: 341-6.
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• Detection and characterization of the Gloeosporium gloeosporioides growth inhibitory compound iturin A from Bacillus subtilis strain KS03. FEMS Microbiol Lett. (2003) 223: 47-51.
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Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.