DLL4 (human):Fc (human) (rec.) (highly active mutant)

Product Description: The Notch ligand delta-like protein 4 (DLL4) is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch1 and Notch4. It is induced by VEGF as a negative feedback regulator and acts to prevent overexuberant angiogenic sprouting, promoting the timely formation of a well differentiated vascular network. DLL4-Notch1 signaling regulates the formation of appropriate numbers of tip cells to control vessel sprouting and branching in the mouse retina.

Alternate Names/Synonyms: Delta-like Protein 4; Delta4

Product type: Protein

Source / Host: HEK 293 cells

Sequence: Extracellular domain of human DLL4 (aa 27-529 plus mutations) are fused at the C-terminus to the Fc portion of human IgG1.

Species Crossreactivity: Human

Specificity: Interacts with human Notch1 with >20 fold increase in affinity relative to WT DLL4 (as confirmed by ELISA).

Molecular Weight: ~80kDa (SDS-PAGE)

TAGs: Fc

Purity: >95% (SDS-PAGE)

Endotoxin: <0.01EU/µg purified protein (LAL test; Lonza).

Formulation: Lyophilized. Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

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