Dolichos biflorus Lectin (DBA) - Pure

Product Description
Pure lectin DBA is isolated from horse gram (Dolichos biflorus) and purified by affinity chromatography. DBA has been used as a general marker of developing renal collecting ducts, which can then be used to establish secretion status in blood group A individuals by hemagglutination inhibition and for detecting blood typing.

DBA is a glycoprotein with a molecular weight of about 120,000 and consists of 4 subunits of equal size. This lectin has an isoelectric point of about pH 5.5 and a carbohydrate specificity toward α-N-acetylgalactosamine, eluting a concentration of 0.2M GalNAc. DBA is over thousand times more active in agglutinating blood group A₁ cells versus A₂ cells, and is used to distinguish these blood types in routine serology applications, and has potential applications in examining tumors which affect the levels of blood group substances.

DBA product comes in a lyophilized powder form and is stable for 3 years from production date when stored at -20 C. Reconstituted product is stable for 6 months at -20 C. Calcium, Manganese, Magnesium, and Zinc are required for binding.

Applications:
Distinguish between A₁ and A₂ blood types

References

• Michael, L., et al. The lectin Dolichos biflorus agglutinin is a sensitive indicator of branching morphogenetic activity in the developing mouse metanephric collecting duct system. J Anat. (2007) 210:89-97.