Tel. : 01 45 33 67 17 / E-mail : coger@coger.fr
AVANTI POLAR LIPIDS , CYGNUS , LARODAN , ADIPOGEN , MYBIOSOURCE , SERVA NORDMARK, BIOWORLD, EDGEBIO , PANREAC APPLICHEM
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The E06 antibody is a murine monoclonal IgM natural antibody that binds to oxidized phospholipid and reacts with oxidized LDL, oxidized HDL and protein covalently modified by oxidized phospholipid. This antibody has been used to probe atherosclerotic plaque in mice, zebrafish, rabbits, monkeys and humans. It also binds to apoptotic cells, but not viable cells, and can be used in a variety of applications, including ELISA, immunohistochemistry and Western blot analysis, as well as for in vivo imaging with MRI techniques.
Avanti now provides high purity E06 from animal-free culture of hybridoma cells. Each batch is
verified for activity and provided with detailed protocols for the above applications.
The E06 antibody was developed by Dr. Joseph Witztum at the University of California, San Diego using apolipoprotein E knock-out mice fed a high-fat diet for seven months.
Antigen:
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Oxidized LDL |
Ig Class:
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Mouse IgM (kappa) |
Specificity:
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E06 recognizes the phosphocholine headgroup of oxidized phospholipid that is present in oxidized LDL and PC-modified BSA. E06 does not bind to normal LDL or unoxidized PC. |
Antibody Source:
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Monoclonal antibody from C57BL/6-derived hybridoma E06. |
Production:
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In vitro cell culture. |
Purification:
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Ultra filtration through 100 KDa cut-off filters. |
Purity:
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≥ 95%. |
Formulation:
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E06 is provided as a 0.2μm sterile-filtered solution in phosphate buffered saline (PBS). |
Recommended Applications:
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ELISA (Sandwich, Direct & Competitive), IHC and WB (see Technical Data Sheet under Downloads tab for more information). |
Storage conditions:
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Store undiluted at 4°C or less. |
Hazardous/Non-hazardous Components:
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This product contains no substances that, at their given concentration, are known to be hazardous to health. Therefore there is no MSDS for this product. |
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