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1 Ea

Ref. 330003S-25µg
AVANTI POLAR LIPIDS

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Détails Produit

E06 mAb-TopFluor®

E06 Mouse Monoclonal Antibody (IgM), TopFluor® labeledAnti-(Oxidized Phospholipid)

The E06-TopFluor® is primarily use for direct ELISA, immune-fluorescence microscopy and flow cytometry.
 
The E06 antibody is a murine monoclonal IgM natural antibody that binds to oxidized phospholipid and reacts with oxidized LDL, oxidized HDL and protein covalently modified by oxidized phospholipid. This antibody has been used to probe atherosclerotic plaque in mice, zebrafish, rabbits, monkeys and humans. It also binds to apoptotic cells, but not viable cells, and can be used in a variety of applications, including ELISA, immunohistochemistry and Western blot analysis, as well as for in vivo imaging with MRI techniques. Avanti now provides high purity E06 from animal-free culture of hybridoma cells. Each batch is verified for activity and provided with detailed protocols for the above applications. The E06 antibody was developed by Dr. Joseph Witztum at the University of California, San Diego using apolipoprotein E knock-out mice fed a high-fat diet for seven months.

Short Name: E06 mAb-TopFluor™

Long Name: E06 Mouse Monoclonal Antibody (IgM), TopFluor labeled Anti-(Oxidized LDL)

Physical State: Saline

Product use:

For binding studies with the E06-TopFluor™ antibody, 96-well Microfluor 1 black U bottom plates (Thermo 7005) were coated with 50µL of antigen (5µg/mL) in PBS containing 0.27 mM EDTA and overnight at 4°C. The wells were washed three times with PBS containing 0.27 mM EDTA. After washing, the plates were blocked with 3% BSA in PBS for 45 min at room temperature. 100µL aliquots of a serial dilution of the E06-TopFluor™ antibody (0.78-400ng/well) in PBS containing EDTA and 1% BSA were added to each well and incubated 1hr at room temperature. The amount of IgM bound to each well was quantified with after 1hr incubation at room temperature with excitation (Ex) at 485 nm and emission (Em) recorded at 520 nm.

 

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