EAH Separopore ® (Agarose) 4B

Applications: EAH-Separopore 4B is used to couple molecules containing carboxyl groups to the matrix for affinity chromatography. It was prepared by covalent coupling of 1, 6-diamino hexane to Separopore 4B via epoxy activation. The free terminal amino group allows for coupling of carboxyl-group containing ligands through amide bond formation by carbodimide-mediated coupling method. Due to the long hydrophilic spacer arm (10-atom), bio-World’s EAH-Separopore 4B is ideal for immobilization of small molecules which are stable in low pH and contain free carboxyl groups.

Technical specifications:

• Matrix active groups: -NH₂ groups
• Matrix: Separopore 4B (agarose beads, 4%)
• Particle size range: 52 - 165 μm
• Matrix activation: Epoxy
• Group to be coupled: -COOH groups
• Chemical linkage of ligand: Stable amide linkage
• Spacer arm: 6-amino caproic acid, 10 atoms
• Ligand density: 7 - 11 μmol amino / ml medium
• Coupling condition: pH 4.5 - 6, Temp: 4 - 25 C
• Storage: 20% ethanol containing 0.9% NaCl
• Storage temperature: 4 - 8 C

References:

• Purification and properties of bovine liver holocarboxylase synthetase. Arch Biochem Biophys. (1994) 313: 8-14.
• Sialidase in rabbit blood. Characterization of sialidase purified from rabbit erythrocyte membrane. Eur J Biochem. (1994) 221: 655-64.
• Isolation of chromosaponin I-specific antibody by affinity chromatography. Biochem Biophys Res Commun. (2003) 302: 587-92.
• Purification of cytochromes P-450(scc) and P-450(17 alpha) by steroid-binding affinity column chromatography. J Biochem Biophys Methods. (2001) 49: 417-31.
• Immobilization of the hydantoin cleaving enzymes from Arthrobacter aurescens DSM 3747. J Biotechnol. (2001) 92: 179-86.
• Immunoaffinity purification of chlorimuron-ethyl from soil extracts prior to quantitation by enzyme-linked immunosorbent assay. J Agric Food Chem. (2001) 49: 1151-7.
• New support for the affinity chromatography of hemoglobin. J Chromatogr B Biomed Sci Appl. (1997) 694: 39-48.
• Affinity-purified Escherichia coli J5 lipopolysaccharide-specific IgG protects neutropenic rats against gram-negative bacterial sepsis. J Infect Dis. (1994) 170: 622-9.
• Refolding of the isolated extracellular domain of the erythropoietin receptor. Biochem Biophys Res Commun. (1994) 200: 482-8.
• Application of poly-L-lysine to purification of leukocyte cathepsin G by affinity chromatography. Chem Pharm Bull (Tokyo). (1992) 40: 1634-6.
• Application of N-carboxyalkyl peptides to the inhibition and affinity purification of the porcine matrix metalloproteinases collagenase, gelatinase, and stromelysin. Arch Biochem Biophys. (1991) 287: 240-9.

Related products:

• ECH-Separopore 4B (SKU # 20181008)
• Aldehyde-Separopore 4B (SKU # 20181058)

Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.