ECH Separopore® (Agarose) 4B

Highlights:

• Affinity matrix for stable carbodiimide-based coupling of ligands containing amino groups.
• Ligands are coupled in a single-step procedure in the presence of a coupling reagent (not provided) which is a water-soluble carbodiimide, such as N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC).
• The matrix provides a 10-atom spacer arm convenient for coupling of small ligands.
• No blocking reaction is necessary following the coupling reaction.
• Ideal for immobilization of small ligands stable in a low pH and containing primary amines.
• Coupling reaction can be carried out in organic solvent.

Technical specifications:

• Matrix active group: -COOH groups
• Matrix: Separopore 4B (agarose beads, 4%)
• Particle size range: 52 - 165 μm
• Molecular weight range: 6 x 10⁴ - 2 x 10⁷
• Group to be coupled: -NH₂ groups
• Chemical linkage of ligand: Stable amide linkage
• Ligand density : 12 - 16 μmol carboxyl groups / ml drained matrix
• Spacer arm: 6-amino caproic acid, 10 atoms
• Coupling conditions pH 4.5 - 6, Temp: 4 C - 25 C, Time: 1.5 - 24 h; organic solvents possible
• Storage: 2 - 8 C
• Supplied as pre-swelled gel suspension in buffered saline

References:

• Serum amyloid P component in mink, a non-glycosylated protein with affinity for phosphorylethanolamine and phosphorylcholine. Amyloid. (2004) 11: 101-8.
• Purification and characterization of a 52-kilodalton immunoglobulin G-binding protein from Streptococcus suis capsular type 2. J Bacteriol. (1995) 177: 3830-6.
• Immunogenicity of the N-glycans of peanut peroxidase. Phytochemistry. (1994) 37: 933-40.
• Rapid purification of protein phosphatase 2A from mouse brain by microcystin-affinity chromatography. FEBS Lett. (1991) 279: 115-8.

Related products:

• Aldehyde-Separopore 4B (SKU # 20181058)
• EAH-Separopore 4B (SKU # 20181007)

Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.