Endotoxin Affisorbent ™ (Polymyxin B) Separopore ® 6B-CL (Columns)

Applications:

bioWorld's Endotoxin Affisorbent™consists of immobilized polymyxin B on Separopore 6B-CL. The polymyxins are a family of antibiotics that contain a cationic cyclopeptide with a fatty acid chain. Polymyxin B neutralizes the biological activity of endotoxins by binding to the lipid A portion of bacterial lipopolysaccharide. The immobilized polymixin B gel is a stable affinity matrix that resists leaching of ligand into the valuable preparation. Affisorbent™ Endotoxin Removing Gel can also be used to remove endotoxins from nucleic acid (DNA) samples. Polymyxin B-Separopore 6B-CL is used in protein chromatography, affinity chromatography and specialty resins. Polymyxin B- Separopore 6B-CL has been used to study periodontitis and endodontic infections as well as obesity and type 2 diabetes. Polymyxin B- Separopore 6B-CL is also used for the removal of endotoxin levels in protein solutions, cell culture media, ascites.

Technical Specifications:

• Ligand: Polymyxin B
• Matrix: Separopore 6B-CL (crosslinked agarose beads, 6%)
• Particle size range: 52 - 165 µm
• Matrix activation: Cyanogen bromide
• Attachment: Amino group
• Binding capacity: 200 - 500 µg LPS from E. coli serotype 0128:B12
• pH stability: 3 - 10
• Supplied as prepacked columns (5 x 1 ml)
• Storage temp: 2 - 8 C

References:

• Mapping of the proinflammatory domains of MspTL of Treponema lecithinolyticum. Microbiology. (2007) 153: 2386-92.
• Human resistin stimulates the pro-inflammatory cytokines TNF-alpha and IL-12 in macrophages by NF-kappaB-dependent pathway. Biochem Biophys Res Commun. (2005) 334: 1092-101.
• Endotoxin contamination contributes to the in vitro cytokine-inducing activity of osteopontin preparations. J Interferon Cytokine Res. (2005) 25: 277-82.
• Upregulation of intercellular adhesion molecule 1 and proinflammatory cytokines by the major surface proteins of Treponema maltophilum and Treponema lecithinolyticum, the phylogenetic group IV oral spirochetes associated with periodontitis and endodontic infections. Infect Immun. (2005) 73: 268-76.
• Heat shock protein 60 from Chlamydia pneumoniae elicits an unusual set of inflammatory responses via Toll-like receptor 2 and 4 in vivo. Eur J Immunol. (2004) 34: 2874-84.
• Recombinant human heat shock protein 60 does not induce the release of tumor necrosis factor alpha from murine macrophages. J Biol Chem. (2003) 278: 22523-9.
• Endotoxin contamination in recombinant human heat shock protein 70 (Hsp70) preparation is responsible for the induction of tumor necrosis factor alpha release by murine macrophages. J Biol Chem. (2003) 278: 174-9.
• Antigenic characterization of the fish pathogen Flavobacterium psychrophilum. Appl Environ Microbiol. (2001) 67: 750-9.
• Phosphatidic acid synthesis in mitochondria. Topography of formation and transmembrane migration. J Biol Chem. (1999) 274: 29786-90.
• Peptidoglycan fragments decrease food intake and body weight gain in rats. Infect Immun. (1994) 62: 3276-81.
• Purification of Chlamydia psittaci antigen by affinity chromatography on polymyxin B agarose for use in the enzyme-linked immunosorbent assay (ELISA). Zentralbl Bakteriol. (1992) 277: 436-45.
• Polymyxin B is a selective and potent antagonist of calmodulin. Eur J Pharmacol. (1991) 207: 17-22.
• Synergistic effect of coumarin (1,2 benzopyrone) and endotoxin in the induction of human interleukin-1. Clin Exp Immunol. (1991) 84: 317-23.
• Production of antibody to lipopolysaccharide (LPS) after immunization with a LPS-polymyxin B-agarose immunogen. J Appl Bacteriol. (1988) 64: 487-95.
• The influence of lipopolysaccharide content on the apparent B cell stimulating activity of anti-mu preparations. J Immunol Methods. (1988) 110: 63-8.
• Inhibition of Escherichia coli growth and respiration by polymyxin B covalently attached to agarose beads. Biochemistry. (1977) 16: 1642-8.

Related products:

• Endotoxin Affisorbent™ (Polymyxin B)-Separopore 4B-CL (SKU # 20181092)
• Endotoxin Affisorbent™ (Polymyxin B)-Separopore 6B-CL (SKU # 20181121)

Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.