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3 x 10 µg

Ref. AG-40B-0185-3010
ADIPOGEN LIFE SCIENCES

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Neuf 400.00 H.T.
en stock

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Détails Produit

Product Details
Synonyms Fibrinogen-like Protein 1; FGL-1; Hepatocyte-derived Fibrinogen-related Protein 1; HFREP-1; Hepassocin
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence

Mouse FGL1 (aa 23-314) is fused at the N-terminus to the Fc portion of human IgG1 and at the C-terminus to a His-Tag.

Crossreactivity Mouse
Application

Can be used to detect mouse LAG-3 at the surface of cells.

Biological Activity

Should activate mouse LAG-3 (activity test not yet performed).

MW ~68kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test; Lonza).
Concentration After reconstitution:
for 10µg size: 0.1mg/ml
for 100µg size: 1mg/ml
Reconstitution Reconstitute with 100μl sterile water.
Accession Number Q71KU9
Formulation Lyophilized. Contains PBS.
Other Product Data

UniProt link Q71KU9: FGL1 (mouse)

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Description

FGL1 (Fibrinogen-like protein 1; also called Hepatocyte-derived fibrinogen-related protein 1; HFREP-1 or Hepassocin) was initially identified as an overexpressed transcript in hepatocyte carcinoma and as a transcript enriched in regenerating liver. FGL1 is expressed at lower levels in brown and white adipose in the setting of liver injury. A low level expression of FGL1 is also observed in the pancreas. FGL1 is a 34 kDa protein structurally similar to Angiopoietin-like factors 2, 3, 4 and 6, which regulate lipid metabolism and energy utilization. It was proposed that FGL1 is a member of an emerging group of proteins having potential roles in liver metabolism and liver regeneration. Recently, FGL1 has also been shown to be upregulated in human cancers and FGL1 is a major functional ligand of LAG-3. FGL1 interacts with LAG-3 in an MHC-II-independent manner and this interaction involves the FGL1 fibrinogen-like domain and the LAG-3 D1-D2 domain. FGL1-LAG-3 interaction blockade promotes tumor immunity by stimulating T cell expansion and activation. FGL1 forms two disulfide-linked homodimers and also higher molecular weight homooligomers that bind to LAG-3 much better than the dimeric forms. This binding to LAG-3 inhibits T cell responses. The recombinant fusion protein Fc (human):FGL1 (human) (FGL1-Ig) has been shown to function as an efficient ligand of LAG-3.

Product References
  1. Fibrinogen-like Protein 1 Is a Major Immune Inhibitory Ligand of LAG-3: J. Wang, et al.; Cell 176, 334 (2019)