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96 wells

Ref. AG-45B-0011-KI01
ADIPOGEN LIFE SCIENCES

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Neuf 590.00 531.00 H.T.

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Détails Produit

Product description: Inflammasomes are multimeric protein complexes that comprise a sensor (e.g. NLRP3), an adaptor (ASC/Pycard) and the procaspase-1. An inflammasome assembles in response to a diverse range of pathogen-associated or danger-associated molecular patterns (PAMPs or DAMPs). The inflammasome platform leads to activation of caspase-1, which further induces maturation of interleukin-1beta and -18 (IL-1beta and IL-18) through proteolytic cleavage of pro-IL-1beta and pro-IL-18. Activated caspase-1, and also the recently characterized caspase-11 non-canonical inflammasome pathway, also cleave the intracellular gasdermin D, which leads to a particular form of inflammatory cell death called pyroptosis. The gasdermin family members contain N-terminal domains that are capable of forming membrane pores to induce cytolysis, whereas the C-terminal domains of gasdermins function as inhibitors of such cytolysis through intramolecular domain association. Caspase-1 or -11 cleavage of gasdermin D is required for regulation of pyroptosis: upon protease cleavage of the gasdermin N- and C-domain linker, the disruption of the intramolecular domain interaction in the presence of lipids releases the N-domain to assemble oligomeric membrane pores that trigger cell death. Gasdermin D seems to be a key effector in the LPS-induced lethal sepsis.

Alternate Names/Synonyms: Gasdermin Domain-containing Protein 1; Gasdermin-D (C-terminal); GSDMD-CT; Gsdmd

Product Type: Kit

Specificity: Detects full-length and cleaved mouse Gasdermin D (C-terminus) in cell culture supernatants and cell extracts. Does not cross-react with human gasdermin D.

Crossreactivity: Mouse

Sensitivity: 14pg/ml

Range: 15.6 to 1000pg/ml

Sample Type: Cell Culture Supernatant, Cell Lysate

Assay Type: Sandwich

Detection Type: Colorimetric

Short Term Storage: +4°C

Long Term Storage: +4°C

Shipping: BLUE ICE

Use & Stability 12 months after the day of manufacturing. See expiry date on ELISA Kit box.

Handling Advice After standard reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Plate and reagents should reach room temperature before use.

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Manuel Kit