Glycine max lectin (soybean) Separopore ® 4B-CL

Applications:

Composed of four subunits of approximately equal size, soybean agglutinin is a family of closely related isolectins. This glycoprotein has a molecular weight of about 120,000 and an isoelectric point near pH 6.0. SBA preferentially binds to oligosaccharide structures with terminal α- or Beta-linked N-acetylgalactosamine, and to a lesser extent, galactose residues. Binding can be blocked by substitutions on penultimate sugars, such as fucose attached to the penultimate galactose in blood group B substance. SBA has been used in glycoprotein fractionation, histochemical applications and cell sorter analysis.

An important application for soybean agglutinin is the separation of pluripotential stem cells from human bone marrow. Cells fractionated by SBA do not produce graft vs host disease and can be used in bone marrow transplantation across histocompatibility barriers (references available upon request). It should be noted that some forms of SBA seem to be excellent in separating human cells while others are better for cells of other species.

Soybean Agglutinin isolated from Glycine max (soybean) seeds is coupled to heat stable, crosslinked 4% agarose beads with a molecular weight exclusion limit of about 2 x 10⁷ used as the solid-phase matrix, to which the lectin is covalently bound. The attachment of the lectin to the solid phase is carefully controlled in order to preserve the activity of the lectin as well as to minimize conformational changes of the bound lectin.

Technical Specifications

• Ligand: Glycine max (soybean) Agglutinin
• Matrix: Separopore 4B-CL (crosslinked agarose, 4%)
• Particle size range: 52 - 165 µm
• Exclusion limit: Approximately 2 x 10⁷
• Ligand density: 2 - 4 mg lectin / ml drained gel
• Binding Capacity: 4.5 mg protein / mL drained gel
• Supplied as Suspension in 0.01M phosphate buffered saline containing 50% glycerol, 0.01M N-acetyl-D-galactosamine and 0.02% sodium azide
• Storage: 2 - 8 C

References:

• Affinity-capture tandem mass spectrometric characterization of polyprenyl-linked oligosaccharides: tool to study protein N-glycosylation pathways. Anal Chem. (2008) 80: 5468-75.
• Evidence for indirect involvement of the H-2Kb gene in the regulation of natural killer sensitivity of BL6 melanoma cells. Nat Immun. (1994) 13: 1-14.
• Increased sensitivity to TNF-mediated cytotoxicity of BL6 melanoma cells after H-2Kb gene transfection. J Immunol. (1993) 151: 3467-77.
• Characterization and partial purification of a plasma membrane receptor for Bacillus thuringiensis var. kurstaki lepidopteran-specific delta-endotoxin. J Cell Sci. (1986) 83: 89-101.

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Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.