GTP Separopore® (Agarose) 4B-CL

Applications:

GTP-Separopore is used for purification of GTP-dependent enzymes and proteins that bind nucleotides and related molecules. The GTP-Separopore 4B-CL resin comprises GTP attached to agarose beads via its γ-phosphate. A long hydrophilic spacer (14-atom) is used to minimize unwanted hydrophobic interactions and to facilitate unhindered interactions with biomolecules. Linkage of GTP via the terminal phosphate means that the immobilized ligand cannot be removed by phosphatases in crude extracts. Affinity resins such as GTP-Separopore 4B-CL are been widely used for the purification of enzymes and other proteins that bind nucleotides and related molecules.

Technical specifications:

• Ligand: Guanosine 5'-triphosphate (GTP)
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 µm
• Molecular weight range: 6 x 10⁴ - 2 x 10⁷
• Ligand density: 8 - 12 mM GTP / ml drained gel
• pH stability: 4 - 9
• Flow specifications: 70 - 140 cm / h
• Supplied as lyophilized powder stabilized with lascose. 1 g will swell aproximately 5-7 ml.

References:

• Purification and characterization of recombinant human soluble guanylate cyclase produced from baculovirus-infected insect cells. Protein Expr Purif. (2009) 65: 133-9.
• Intramolecular activation mechanism of the Dictyostelium LRRK2 homolog Roco protein GbpC. J Biol Chem. (2008) 283: 30412-20.
• High yield purification of soluble guanylate cyclase from bovine lung. Protein Expr Purif. (2008) 60: 58-63.
• Binding analyses for the interaction between plant virus genome-linked protein (VPg) and plant translational initiation factors. Biochimie. (2006) 88: 329-40.
• Characterization of purified rat testicular transglutaminase and age-dependent changes of the enzyme activities. Int J Biochem Cell Biol. (2005) 37: 386-96.
• GTP cyclohydrolase I: purification, characterization, and effects of inhibition on nitric oxide synthase in nocardia species. Appl Environ Microbiol. (2003) 69: 7507-13.
• Potential role for high and low molecular weight tissue transglutaminases in transforming mammalian cell properties. Curr Drug Targets Immune Endocr Metabol Disord. (2001) 1: 223-32.
• A sucrose-binding protein homologue from soybean exhibits GTP-binding activity that functions independently of sucrose transport activity. Eur J Biochem. (2002) 269: 3998-4008.
• GTP-binding properties of the membrane-bound form of porcine liver annexin VI. Acta Biochim Pol. (2001) 48: 851-65.
• GTP-dependent permeabilized neutrophil secretion requires a freely diffusible cytosolic protein. J Cell Biochem. (2000) 80: 37-45.
• A new member of alpha 1-adrenoceptor-coupled G alpha h (transglutaminase II) family in pig heart: purification and characterization. Exp Mol Med. (1998) 30: 81-6.
• The core domain of the tissue transglutaminase Gh hydrolyzes GTP and ATP. Biochemistry. (1997) 36: 11655-64.
• The signal recognition particle receptor alpha subunit of the hyperthermophilic archaeon Acidianus ambivalens exhibits an intrinsic GTP-hydrolyzing activity. Biochim Biophys Acta. (1997) 1335: 218-30.
• Expression of the Arabidopsis G-protein GP alpha1: purification and characterisation of the recombinant protein. Plant Mol Biol. (1997) 33: 723-8.
• Mutation of lysine residues in the nucleotide binding segments of the poliovirus RNA-dependent RNA polymerase. J Virol. (1996) 70: 8564-70.
• Purification and cloning of the GTP cyclohydrolase I feedback regulatory protein, GFRP. J Biol Chem. (1996) 271: 19743-51.
• Phospholipase D regulation by a physical interaction with the actin-binding protein gelsolin. Biochemistry. (1996) 35: 5229-37.
• Specific cleavage of recombinant protein tau3 between valine-220 and tyrosine-221 (val-309 and tyr-310 of tau4) by a double-stranded DNA-stimulated protease. Biochem Biophys Res Commun. (1996) 221: 248-53.
• Mutational inactivation of the catalytic domain of guanylate cyclase-A receptor. Hypertension. (1995) 25: 694-8.
• Microtubule severing by elongation factor 1 alpha. Science. (1994) 266: 282-5.

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Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.