Hippeastrum hybrid Lectin (HHL) - Pure

Product Description
Pure lectin HHL isolated from amaryllis bulbs, Hippeastrum hybrid and purified by affinity chromotography. HHL consists of four subunits of identical size. HHL has a molecular weight of 50,000 Da and isoelectric points of pH 4.7 to 5.1. HHL only binds α-mannose residues, (α-1,3) and (α-1,6) linked mannosyl units, as well as some yeast galactomannans, and elutes 0.2M of MeαMan. HHL has a blood group specificity for rabbit.

Study shows when treatment of the linear α-1,3-mannan with periodate, resulting in oxidation of the terminal, nonreducing mannosyl group, reactivity with HHL was not reduced. Oligosaccharides containing either 1,3- or 1,6-linked mannosyl units are good inhibitors of HHL-mannan precipitation system. Observations suggests that HHL reacts not only with terminal but also with internal α-D-mannosyl residues. Lectin appears to possess extended binding sites complementary to at least three 1,6-linked α-mannosyl units. Those containing α-1,3- or α-1,6-mannosyl residues were retarded on immobilized HHL column.

This product comes in a lyophilized powder form and is stored at -20 C. No metals ions are required for binding.

Applications:
Glycobiology

References

• Kaku H. Carbohydrate-binding specificity of the daffodil (Narcissus pseudonarcissus) and amaryllis (Hippeastrum hybr.) bulb lectins. Arch. Biochem. Biophys. (1990) 279(2):298-304.