HpARI (Alarmin Release Inhibitor) (rec.) (His)

Product Description: HpARI is a protein secreted by the mouse parasite Heligmosomoides polygyrus. The mature protein HpARI, containing three predicted Complement Control Protein (CCP)-like modules (also known as Short Consensus Repeats (SCRs) or sushi-domains), suppresses type 2 (allergic) immune responses through interference in the interleukin-33 (IL-33) pathway. During cell damage, HpARI gains access to the nucleus of necrotic cells, where it binds directly to IL-33 and nuclear DNA, preventing secretion and binding of IL-33 to its receptor. HpARI is a new type of reagent to prevent IL-33-mediated pathology.

Alternate Names/Synonyms: Heligmosomoides Polygyrus Alarmin Release Inhibitor

Product type: Protein

Source / Host: HEK 293 cells

Sequence: Heligmosomoides polygyrus protein HpARI (aa 17-251) is fused at the C-terminus to a His-tag.

Species Crossreactivity: Human, Mouse

Specificity: Binds to human and mouse IL-33.

Molecular Weight: ~40kDa

TAGs: His

Purity: >90% (SDS-PAGE)

Endotoxin: <0.01EU/µg purified protein (LAL test; Lonza).

Concentration: After reconstitution: 1mg/ml

Formulation: Lyophilized. Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

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