IL-33 (mouse) (rec.) (His)

Product Description: Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kDa human IL33 is converted by CASP1 to a 18kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3)/ERK2 (MAPK1), p38 (MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.

Alternate Names/Synonyms: Interleukin-33; IL-1F11; NF-HEV

Product type: Protein

Source / Host: E. coli

Sequence: Mouse IL-33 (aa 109-266) is fused at the C-terminus to a His-tag.

Species Crossreactivity: Human, Mouse

Specificity: Binds to mouse and human ST2.

Biological Activity: Activates mouse and human ST2-dependent NF-kappaB pathway.

Molecular Weight: ~20kDa (SDS-PAGE)

TAGs: His

Purity: >90% (SDS-PAGE)

Endotoxin: <1EU/µg purified protein (LAL test; Lonza).

Formulation: Liquid. 0.2µm-filtered solution in 55mM TRIS-Cl, pH 8.2, containing 150mM NaCl.

Handling Advice: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 2 years after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

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