Iron Colorimetric Assay Kit (Ferrozine Method)

Product description: Iron is a mineral (functioning as an enzyme cofactor) that plays an essential role in many biological processes. It is essential to nearly all known organisms. As a transition element it can form a range of oxidation states, the most common being Fe2+ (or ferrous iron) and Fe3+ (or ferric iron). Ingested iron is mainly absorbed in the form of Fe2+. The trivalent form and the heme-bound Fe2+-component of iron is reduced by vitamin C. Before passing into the plasma, it is oxidized by ceruloplasmin to Fe3+ and bound to transferrin to form a transferrin-iron complex. Iron is generally stored in the centre of metalloproteins, in the heme complex, and in oxygen carrier proteins. Iron-containing proteins participate in many reactions, often utilizing transitory changes in the oxidation state of iron to carry out chemical reactions. Iron is important for redox reactions, oxygen transport (e.g. hemoglobin), short-term oxygen storage (e.g. myoglobin) and energy generation. Iron deficiency has many adverse consequences, including anemia, hemochromatosis, chronic renal disease and in children, behavioral and learning disorders. Iron excess is toxic to the body, harming the heart, liver, skin, pancreatic islet beta cells, bones, joints, and pituitary gland. Maintaining proper iron balance is essential for maintaining homeostasis and health.

Product Type: Kit

Specificity: Specific to Fe2+ and Fe3+.

Crossreactivity: All

Kit Contains: 1 x 20 ml R-1 Buffer (ready to use) 1 x 0.8 ml R-2 Chelate color (ready to use) 1 x 4 ml STD Iron standard 200 µg/dL (ready to use)

Range: 5 - 1'000µg/dL

Sample Type: Cell Lysate, Plasma, Saliva, Serum, Tissue Supernatant, Urine

Detection Type: Colorimetric

Other data: This MC-Reagent Iron Assay Kit is a direct colorimetric assay based on the FerroZine® method without deproteinization of the sample. A weak acid buffer dissociates iron from the transferrin-iron complex and is reduced by a reductant (Ferric >> Ferrous). Ferrous ions form a complex with the chromogen Ferrozine. The color intensity is proportional to the iron concentration in the sample. Absorbance of the Fe2+-complex is measured at 560nm. Wavelength range of sensitivity: 540 ~ 580nm. Features of this Assay: Quick & Easy to use • Species independent • Highly sensitive, stable and suitable for high-throughput testing • No toxic substances • For cell lysates, serum, plasma and wide variety of biological samples

Short Term Storage: +4°C

Long Term Storage: +4°C

Shipping: BLUE ICE

Use & Stability 12 months after the day of manufacturing. See expiry date on ELISA Kit box.

Handling Advice Do not freeze.

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