KillerTRAIL™ (R1 specific), Soluble (human) (rec.)

Product Description: TRAIL-R1 is a receptor for the cytotoxic ligand TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappaB.

Alternate Names/Synonyms: TRAIL; Apo-2L; TNFSF10; CD253

Product type: Protein

Source / Host: E. coli

Sequence: The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide and contains aa substitutions, which generate a binding specificity towards TRAIL receptor 1 (TRAIL-R1; DR4).

Species Crossreactivity: Human

Specificity: Binds to human TRAIL receptor 1 (DR4), but not human TRAIL-R2 (DR5). Binding to TRAIL-R3, -R4 and osteoprotegrin (OPG) are not tested.

Biological Activity: Induces apoptosis in a concentration range of 20-500ng/ml of TRAIL-R1 (DR4) expressing cell lines (e.g. RAMOS). It is inefficient to kill via TRAIL-R2 (DR5) in Jurkat cells. The activity depends on the relative cellular surface expression of TRAIL-R1 compared to other death-signaling TRAIL-Rs. Concentrations of KillerTRAIL™-R1 to induce apoptosis may vary depending on the cell type.Note: This protein does not require a cross-linking enhancer for its potent biological activity.

Molecular Weight: ~26kDa

TAGs: His

Purity: >98% (SDS-PAGE)

Endotoxin: <0.1EU/µg purified protein (LAL test; Lonza).

Concentration: 0.5mg/ml

Formulation: Liquid. In 20mM HEPES, pH 7.9, 120mM NaCl, 0.01% Tween 20, 1% sucrose, 1mM DTT.

Handling Advice: After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.

Short Term Storage: -20°C

Long Term Storage: -80°C

Shipping: DRY ICE

Use & Stability: Once thawed it is recommended to prepare appropriate aliquots and to store them at -80°C. , Stable for at least 1 year after receipt when stored at -80°C.

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