Lactose Separopore® (Agarose) 6B-CL

Applications:

α-Lactose-Separopore 6B-CL is used in protein chromatography, affinity chromatography and carbohydrate matrices. α-Lactose-Separopore 6B-CL has been used to study microneme proteins and host-parasite cell surface receptors. Lactose-Separopore 6B-CL may be used to isolate lactose binding proteins, such as lectins and glycoproteins.

Technical Specifications:

• Ligand: Lactose
• Matrix: Separopore 6B-CL (crosslinked agarose beads, 6%)
• Particle size range: 52 - 165 µm.
• Matrix activation: Expoy
• Matrix attachment: Hydroxyl
• Matrix spacer: 5 atoms
• Binding capacity: 20 - 25 mg lectin from Arachis hypogaea / ml drained gel
• Storage: 2 - 8 C.
• Suspension in 0.15M NaCl, 0.01M sodium phosphate, pH 6.8, containing 0.02% sodium azide

References:

• Isolation of galectin-1 from human platelets: its interaction with actin. Protein J. (2012) 31: 8-14.
• Purification and molecular cloning of a new galactose-specific lectin from Bauhinia variegata seeds. J Biosci. (2008) 33: 355-63.
• Characterization and purification of the discoidin domain-containing protein retinoschisin and its interaction with galactose. Biochemistry. (2008) 47: 9098-106.
• Galectin-3 expression in human papillary thyroid carcinoma. Cancer Genomics Proteomics. (2008) 5: 117-22.
• Vaccination with microneme protein NcMIC4 increases mortality in mice inoculated with Neospora caninum. J Parasitol. (2007) 93: 1046-55.
• The second glutamic acid in the C-terminal CRD affects the carbohydrate-binding properties of recombinant galectins of Haemonchus contortus. Vet Parasitol. (2007) 148: 247-55.
• Amino acid sequence and characterization of C-type lectin purified from the snake venom of Crotalus ruber. Comp Biochem Physiol B Biochem Mol Biol. (2007) 146: 299-306.
• Unexpected different binding of mistletoe lectins from plant extracts to immobilized lactose and N-acetylgalactosamine. Anal Chem Insights. (2007) 2: 43-50.
• Anti-glycosyl antibodies in lipid rafts of the enterocyte brush border: a possible host defense against pathogens. Am J Physiol Gastrointest Liver Physiol. (2005) 289: G1100-7.
• Identification and characterization of a Neospora caninum microneme-associated protein (NcMIC4) that exhibits unique lactose-binding properties. Infect Immun. (2004) 72: 4791-800.
• Isolation and characterization of galectins in the mammalian retina. Invest Ophthalmol Vis Sci. (2001) 42: 2164-72.
• Cloning and characterization of a beta-galactoside-binding protein (galectin) from the gut of the gastrointestinal nematode parasite Haemonchus contortus. Parasitology. (1999) 119: 483-90.
• Processing of preproricin in transgenic tobacco. Protein Expr Purif. (1999) 15: 188-95.
• Actinomyces serovar WVA963 coaggregation-defective mutant strain PK2407 secretes lactose-sensitive adhesion that binds to coaggregation partner Streptococcus oralis 34. Oral Microbiol Immunol. (1998) 13: 337-40.
• Murine microglial cells express functionally active galectin-3 in vitro. J Neurosci Res. (1998) 51: 49-57.
• Identification of a 95 kDa putative adhesin from Actinomyces serovar WVA963 strain PK1259 that is distinct from type 2 fimbrial subunits. Microbiology. (1997) 143: 835-46.
• Identification of galectin-3 as a factor in pre-mRNA splicing. Proc Natl Acad Sci U S A. (1995) 92: 1213-7.
• Purification and characterization of the N-terminal domain of galectin-4 from rat small intestine. FEBS Lett. (1995) 359: 169-72.
• Characterization of isolectins in Tetracarpidium conophorum seeds (Nigerian walnut). Glycoconj J. (1994) 11: 299-303.
• Purification and properties of a novel beta-galactosidase or exo-(1-->4)-beta-D-galactanase from the cotyledons of germinated Lupinus angustifolius L. seeds. Planta. (1994) 192: 502-11.
• ATP-dependent translocation of ricin across the membrane of purified endosomes. J Biol Chem. (1993) 268: 23661-9.
• Peanut lectin: a mitogen for normal human colonic epithelium and human HT29 colorectal cancer cells. J Natl Cancer Inst. (1992) 84: 1410-6.

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Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.