Lens culinaris Lectin (LCA/LCH) - Separopore® 4B

Lens culinaris agglutinin has a molecular weight of approximately 49,000 and is composed of four subunits - two of about 17,000 daltons and two of 8,000 daltons. The isoelectric point of LCA is approximately pH 8.5. LCA recognizes sequences containing a-linked mannose residues. Like other mannose specific lectins, divalent cations such as calcium and manganese are required for sugar binding activity.

Lens Culinaris Agglutinin isolated from Lens culinaris (lentil) seeds is coupled to heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 used as the solid-phase matrix to which the lectin is covalently bound. The attachment of the lectin to the solid phase is carefully controlled in order to preserve the activity of the lectin as well as to minimize conformational changes of the bound lectin.

Specifications

• Form: 50% slurry
• Matrix: cross-linked 4% beaded agarose
• Ligand: Lens culinaris agglutinin
• Binding Capacity: 3 mg mannosyl glycoprotein/mL gel
• Storage: 2-8 C
• Supplied: HEPES/NaCl/Mannose buffer + Na azide

Usage : bioWORLD`s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.