Maackia amurensis Lectin (MAA/MAL II) - Pure

Product Description
Hemagglutinin isoelectin (MAL II) is isolated from Amur seeds, Maackia amurensis, and is a glycoprotein. This lectin has a molecular weight of 130,000 Da and is a dimer that consists of two subunits. MAL II has an isoelectric point of pH 4.7 and a carbohydrate specificity for Neu5Acα2,3GalBeta1,4GlcNA.

The amino acid residues involved in the sugar-binding of Maackia lectins are different than those of leguminous lectins having other specificities. Maackia amurensis lectin II has non-specific blood group specificity and therefore agglutinates all types of human erythrocytes. Studies show MAL II has a high affinity for Ser/Thr-linked oligosaccharides containing sialic acid residues linked α2,3 to galactosyl residues. MAL II can also bind unsialylated structures, such as glycans containing SO₄-3-GalBeta1-3GalNAc.

This product comes in lyophilized powder form and is stored at -20 C. No metal ions required for binding.

Applications:
Suitable for use in ELISA

References

• Geisler, C. and Jarvis, D. Effective glycoanalysis with Maackia amurensis lectins requires a clear understanding of their binding specificities. Glycobiology. (2011) 21(8):988-993.
• Yamamoto, K., et al. Sialic acid-binding motif of Maackia amurensis lectins. J Biochem. (1997) 121:756-761.