N-Acetyl-galactosamine Separopore® (Agarose) 4B-CL

Applications:

N-Acetyl-D-galactosamine-Separopore 4B-CL is an agarose affinity chromatography matrix used to purify carbohydrate-binding proteins such as membrane receptors, and also lectins with affinity to N-Acetyl-galactosamine. N-Acetyl-D-galactosamine-Separopore 4B-CL has been used to study periodontal disease and to facilitate the design of potent small-molecule ice recrystallization inhibitors. N-Acetyl-D-galactosamine-Separopore 4B-CL has also been used to demonstrate a molecular shuttle between extracellular and cytoplasmic space allowing for monitoring of GAG biosynthesis. -

Technical Specifications:

• Ligand: N-Acetyl-galactosamine
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 µm
• Matrix activation: Epoxy
• Matrix attachment: Hydroxyl
• Matrix spacer: 12 atoms
• Binding capacity: ≥6 mg lectin from Glycine max / ml drained gel
• Storage: 2 - 8 C
• Supplied as Suspension in 0.15 M PBS containing 0.03% thimerosal

References:

• Deactivation of Soybean Agglutinin by Enzymatic and Other Physical Treatments. J Agric Food Chem. (2010) (In Press).
• Characterization and purification of the discoidin domain-containing protein retinoschisin and its interaction with galactose. Biochemistry. (2008) 47: 9098-106.
• Isolation and characterization of the localized adherence factor of enteropathogenic Escherichia coli. Infect Immun. (1988) 56: 2979-83.

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Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.