Netrin-1 (human) (rec.)

Product Description: Netrin-1 controls guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Netrin-1 is also Involved in tumorigenesis by regulating apoptosis. Netrin-1 promotes atherosclerosis by retaining macrophages in the artery wall. Netrin-1 also governs induced pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by limiting apoptosis mediated by Netrin-1 receptors DCC or UNC5b.

Alternate Names/Synonyms: NTN1

Product type: Protein

Source / Host: HEK 293 cells

Sequence: Human netrin-1 (aa 25-604) is fused at the C-terminus to a FLAG®-tag.

Species Crossreactivity: Human, Mouse, Rat

Specificity: Binds to human, mouse and rat UNC5B.

Biological Activity: Induces pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by inhibiting apoptosis mediated by the receptors DCC or UNC5B (at 150ng /ml). Induces axon outgrowth.

Molecular Weight: ~80kDa (SDS-PAGE)

TAGs: FLAG

Purity: >95% (SDS-PAGE)

Endotoxin: <0.01EU/µg purified protein (LAL test; Lonza).

Concentration: 10µg vial: 0.1mg/ml after reconstitution.100µg vial: 1mg/ml after reconstitution.

Formulation: Lyophilized. Contains PBS plus protein stabilizer. No BSA included. The protein stabilizer shows no interference with protein activity.

Handling Advice: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

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