OnePASS™ Blue Separopore ® (Agarose) 6B-CL

Applications:

Cibacron Blue 3GA-Separopore 6B-CL is an agarose conjugate in saline suspension used to searate and purify a variety of proteins. The ligand Cibacron Blue F3G-A is covalently coupled to Separopore via chlorotriazine ring. The structure of the blue dye makes it a very versatile tool for separating many proteins, e.g. albumin, interferon, lipoproteins and blood coagulation factors. Blue-Separopore 6B-CL is used for purification of albumin, enzymes (including NAD⁺ and NADP⁺), coagulation factors, interferons and related proteins. Due to a broad spectrum of the affinity, this product can be used as a general purpose protein purification tool. The cross-linked matrix provides a stable, rigid medium even in the presence of dissociating agents.

Technical Specifications:

• Ligand: Cibacron Blue F3G-A
• Matrix: Separopore 6B-CL (crosslinked agarose, 6%)
• Particle size range: 52 - 165 µm
• Matrix activation: Triazine coupling
• Coupling conditions: pH 9 - 10
• Ligand density: 8 µmol Cibacron Blue/ml drained gel
• Binding capacity: > 15 - 20 mg bovine serum albumin/ml drained gel
• pH stability: 3 - 13
• Chemical stability: Stable to all commonly used aqueous buffers and additives such as 8M urea and 6M guanidine hydrochloride.
• Physical stability: Negligible volume variation due to changes in pH or ionic strength.
• Supplied as Suspension in 0.5M NaCl containing 0.02% thimerosal
• Storage: 2 - 8 C

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