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20 µg

Ref. SBB-US0012-C020
SOUTH BAY BIO

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Neuf 381.00 H.T.

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Détails Produit

Product Description: p53 acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. E3 ligase MDM2 mediated ubiquitin conjugation of p53 and subsequent p53 down-regulation via proteasomal degradation interrupts this crucial role, contributing to tumorogenesis (and cancer).

Alternate Names/Synonyms: Cellular Tumor Antigen p53; Tumor Suppressor p53; TP53

Product type: Protein

Source / Host: E. coli

Sequence: Human p53 (aa 2-393) fused to a N-terminal His-tag, ubiquitinated by MDM2, and subsequently purified from conjugation-reaction proteins/enriched post-conjugation.

Species Crossreactivity: Human

Molecular Weight: >40kDa. Western blot analysis shows multiple states of poly-ubiquitination, and deconjugation with USP7 shows purified ubiquitinated p53 can function as a native substrate.

TAGs: His

Purity: >95% (SDS-PAGE)

Concentration: Lot dependent.

Formulation: Liquid. In 50mM HEPES pH 7.5, 500 mM sodium chloride, 10% glycerol, 2mM TCEP.

Handling Advice: Aliquot to avoid freeze/thaw cycles.

Short Term Storage: -80°C

Long Term Storage: -80°C

Shipping: DRY ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -80°C.

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