Phaseolus vulgaris Lectin (PHA-E+L) - Pure

Product Description
This lectin (PHA-E+L) is isolated from red kidney beans, Phaseolus vulgaris, and consists of the two subunit lectins PHA-E and PHA-L before separation of erythroagglutinin and leucoagglutinin. It has a molecular weight of 126,000 Da and is made up of four subunits. This lectin has an isoelectric point of pH 5.2 - 6.2 and a carbohydrate specificity for complex oligosaccharides, and elutes a concentration of 0.2M of bovine thyroglobulin or acetic acid. PHA E+L agglutinates blood group A (-SA).

PHA E+L (also known as PHA-P, P standing for phytohemagglutinin) is a crude preparation that contains all five of the isolectins. PHA-P is not soluble in distilled water and dilute buffers containing 10 - 15 mM NaCl will solubilize it.

This lectin has the potential to induce closer contacts between adjacent cell membranes, and has been successfully used for membrane-induced fusion in human oocytes, bovine oocyt, and caprine oocytes.

This product comes in a lyophilized powder form and is stable for more than three years from production date when stored below -20 C. Calcium and manganese ions are required for binding activity.

Applications:
Lateral flow erythrocyte separation
Mitogenic activities

References

• Liener E., et al. The lectins - properties, functions and applications in biology and medicine. (1986).
• Movafagh, A., et al. The significance application of indigenous phytohemagglutinin (PHA) mitogen on metaphase and cell culture procedure. Iran J Pharm Res. (2011) 10(4):895-903.