Pisum sativum Lectin (PSA/PSL) - Pure

Product Description
Pisum sativum lectin/agglutinin (PSA) is isolated from peas and purified by affinity chromatography. PSA has a molecular weight of around 53,000 Da and is a heterodimer that consists of four subunits. It has an isoelectric point of pH 6.0 - 6.7 and a carbohydrate specificity towards α-mannose and α-glucose, that elutes a concentration of 0.2M of α-methylglucoside + α-methylmannoside. It’s hemagglutinating activity can be inhibited by glucose, mannose, and sucrose.

The N-terminal sequence of the lectin has shown some degree of homology compared to lectins from other legume species. Pisum sativum lectin has also shown mitogenic effect toward murine splenocytes and could inhibit activity of HIV-1 reverse transcriptase.

This product comes in a lyophilized powder form and is stable for more than three years from production date when stored below -20 C. Metal ions calcium and manganese are required for binding activity.

Applications:
Agglutination studies
Model system of how proteins recognize carbohydrates

References

• Nq, T., et al. Purification and characterization of a lectin from green split peas (Pisum sativum). Appl biochem biotechnol. (2015) 177(6):1374-85.