Protein A Red Separopore ® (Agarose) 4B-MB (Macrobeads)

Applications:

Protein-A Red Separopore 4B-MB functions the same as conventional affinity beads, but have enhanced visibility due to red colored Separopore macrobeads. The red colored beads help provide visual monitoring to avoid the accidental loss of beads during pull-down in immunoprecipitation (IP) procedures, thereby increasing efficiency and yield.Affinity matrix with immobilized Protein-A by CNBr activation coupling method.

• For immunoprecipitation and co-immunoprecipitation experiments.
• Reliable tool to purify monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants.

Technical Specifications

• Ligand: Recombinant Protein-A (42 kDa)
• Matrix: Red Separopore 4B-MB (agarose macrobeads, 4%)
• Particle size range: 150 - 265 µm
• Matrix activation: Cyanogen bromide
• Ligand density: ~ 3 mg Protein A/ml medium
• Binding capacity: ~ 20mg human IgG / ml drained gel
• Molecular weight range: 6 x 10⁴ - 2 x 10⁷
• pH stability: 3 - 9 (for use), 2 - 10 (for cleaning)
• Storage: 2 - 8 C
• Supplied as suspension in 0.5M NaCl, 0.02% sodium azide

References:

• Identification of a novel storage glycine-rich peptide from guava (Psidium guajava) seeds with activity against Gram-negative bacteria. Peptides. (2008) 29: 1271-9.
• An antifungal peptide from passion fruit (Passiflora edulis) seeds with similarities to 2S albumin proteins. Biochim Biophys Acta. (2006) 1764: 1141-6.
• Toxicity to cotton boll weevil Anthonomus grandis of a trypsin inhibitor from chickpea seeds. Comp Biochem Physiol B Biochem Mol Biol. (2005) 140: 313-9.
• A ribonuclease with antimicrobial, antimitogenic and antiproliferative activities from the edible mushroom Pleurotus sajor-caju. Peptides. (2004) 25: 11-7.
• Contamination of ribosome inactivating proteins with ribonucleases, separated by affinity chromatography on red sepharose. Prep Biochem Biotechnol. (2003) 33: 101-11.
• Molecular cloning and characterization of the gene coding for azoreductase from Bacillus sp. OY1-2 isolated from soil. J Biol Chem. (2001) 276: 9059-65.
• A carbonyl reductase-catalyzing reduction of N3-phenacyluridine in rabbit liver. Biol Pharm Bull. (2000) 23: 917-21.
• Preparation of highly purified momordin II without ribonuclease activity. Life Sci. (1999) 65: 1485-91.
• The heparin/heparan sulfate-binding site on apo-serum amyloid A. Implications for the therapeutic intervention of amyloidosis. J Biol Chem. (1999) 274: 7172-81.
• Enzymological characteristics of the hyperthermostable NAD-dependent glutamate dehydrogenase from the archaeon Pyrobaculum islandicum and effects of denaturants and organic solvents. Appl Environ Microbiol. (1998) 64: 2152-7.
• Purification and partial characterization of an indomethacin hydrolyzing enzyme from pig liver. Pharm Res. (1996) 13: 1327-35.
• Rapid purification of native SecA from Escherichia coli: development of a new affinity chromatography procedure. Curr Microbiol. (1994) 29: 323-9.
• Purification and characterization of an autocrine migration factor for vascular smooth muscle cells (SMC), SMC-derived migration factor. J Biol Chem. (1993) 268: 13301-8.
• A novel NADPH-dependent cytosolic 3,5,3'-triiodo-L-thyronine-binding protein (CTBP; 5.1S) in rat liver: a comparison with 4.7S NADPH-dependent CTBP. Endocrinology. (1991) 129: 1701-8.
• A new family of small (5 kDa) protein inhibitors of insect alpha-amylases from seeds or sorghum (Sorghum bicolar (L) Moench) have sequence homologies with wheat gamma-purothionins. FEBS Lett. (1991) 279: 101-4.
• Purification of cytosolic 3,5,3'-triiodo-L-thyronine(T3)-binding protein(CTBP) which regulates nuclear T3 translocation. Biochem Biophys Res Commun. (1991) 174: 1084-9.
• Purification and characterization of rho-crystallin from Japanese common bullfrog lens. J Biol Chem. (1990) 265: 9914-23.
• Purification and characterization of an insect alpha-amylase inhibitor/endochitinase from seeds of Job's Tears (Coix lachryma-jobi). Biochim Biophys Acta. (1989) 999: 260-6.
• IL-2 receptor(p55)/Tac-inducing factor. Purification and characterization of adult T cell leukemia-derived factor. J Immunol. (1988) 140: 2614-20.

Related products:

• Avidin-Separopore 6B (SKU # 20181060)
• Calmodulin-Separopore 4B (SKU # 20181001)
• E. coli Protein-Separopore 4B (SKU # 20181006)
• Gelatin-Separopore 4B (SKU # 20181012)
• Heparin-Separopore 4B (SKU # 20181013)
• KLH-Separopore 4B (SKU # 20181016)
• Papain-Separopore 4B (SKU #20181027)
• Protein A-Separopore 4B-CL (SKU # 20181029)
• Protein A-Separopore 4B-CL (Lyophilized) (SKU # 20181028)
• Protein A Blue-Separopore 4B-MB (Macrobeads) (SKU # 20181042)
• View-IP™ Protein-A Red Separopore 4B-CL (SKU # 20181041)
• Protein G-Separopore 4B-CL (SKU # 20181030)
• Streptavidin-Separopore 4B (SKU # 20181037)
• Ubiquitin-Separopore 4B (SKU # 20181040)

Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.