Protein A Separopore ® 4B-CL

Applications:

Protein A binds specifically to F_c regions of many mammalian immunoglobulins. Protein A- conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological fluids. Protein A-Separopore 4B-CL is used for purification and/or fractionation of IgG subclasses. It is a reliable tool to purify monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants. This is also commonly used to capture immune complexes generated in immunoprecipitation (IP) experiments.

Technical Specifications

• Ligand: recombinant Protein A from E. coli
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 µm
• Matrix activation: Cyanogen bromide
• Molecular weight range: 6 x 10⁴ - 2 x 10⁷
• Ligand density: ~3 mg Protein A / ml drained gel
• Binding capacity: ~ 20 mg human IgG / ml drained gel
• pH stability: 3 - 9 (for use), 2 - 10 (for cleaning)
• Storage: 2 - 8 C.
• Supplied as 50% slurry in PBS with sodium azide

References:

• Expression, purification and characterization of a human single-chain Fv antibody fragment fused with the Fc of an IgG1 targeting a rabies antigen in Pichia pastoris. Protein Expr Purif. (2012) 86: 75-81.
• Leishmania (Viannia) braziliensis nucleoside triphosphate diphosphohydrolase (NTPDase 1): Localization and in vitro inhibition of promastigotes growth by polyclonal antibodies. Exp Parasitol. (2012) 132: 293-9.
• The design of protein-imprinted polymers as antibody substitutes for investigating protein-protein interactions. Biomaterials. (2012) 33: 3344-52.
• Improved efficacy by using the pTnT-rhtTG plasmid for the detection of celiac disease specific tissue transglutaminase autoantibodies in radioligand binding assays. Scand J Clin Lab Invest. (2011) 71: 701-4.
• A novel triple mix radiobinding assay for the three ZnT8 (ZnT8-RWQ) autoantibody variants in children with newly diagnosed diabetes. J Immunol Methods. (2011) 371: 25-37.
• Antibody purification by affinity chromatography based on small molecule affinity ligands identified by SPR-based screening of chemical microarrays. J Chromatogr A. (2011) 1218: 4649-59.
• Occurrence of a conserved domain in ATP diphosphohydrolases from pathogenic organisms associated to antigenicity in human parasitic diseases. Dev Comp Immunol. (2011) 35: 1059-67.
• Expression of a recombinant chimeric protein of hepatitis A virus VP1-Fc using a replicating vector based on Beet curly top virus in tobacco leaves and its immunogenicity in mice. Plant Cell Rep. (2011) 30: 1513-21.
• Chromatographic behaviour of monoclonal antibodies against wild-type amidase from Pseudomonas aeruginosa on immobilized metal chelates. Biomed Chromatogr. (2011) 25: 1327-37.
• BioVyon Protein A, an alternative solid-phase affinity matrix for chromatin immunoprecipitation. Anal Biochem. (2011) 412: 183-8.
• Frontal affinity chromatography analysis of constructs of DC-SIGN, DC-SIGNR and LSECtin extend evidence for affinity to agalactosylated N-glycans. FEBS J. (2010) 277: 4010-26.
• Large scale purification and characterization of recombinant human autotaxin/lysophospholipase D from mammalian cells. BMB Rep. (2010) 43: 541-6.
• Binding of lac repressor-GFP fusion protein to lac operator sites inserted in the tobacco chloroplast genome examined by chromatin immunoprecipitation. Nucleic Acids Res. (2010) 38: e145.
• Synthetic rabbit-human antibody conjugate as a control in immunoassays for immunoglobulin M specific to hepatitis E virus. Virol J. (2010) 7: 101.
• Trace-level detection of atrazine using immuno-chemiluminescence: dipstick and automated flow injection analyses formats. J AOAC Int. (2010) 93: 28-35.
• Different in vitro toxicities of structurally similar type I ribosome-inactivating proteins (RIPs). Toxicol In Vitro. (2010) 24: 1176-82.
• Metal dependent hydrolysis of Beta-casein by sIgA antibodies from human milk. J Mol Recognit. (2011) 24: 45-59.

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Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.