Q Separopore® 4B (Fast Flow)

Usage:

bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Applications:

Q Separopore 4B is crosslinked high gel strength bio agarose matrix for gel filtration. The matrix is coupled with quaternary ammonium cation to provide elution specificity through charge interactions. Capillary electrophoresis as well as ion chromatography are two of the major analytical techniques this product is employed in. It is ideal for both analytical and preparative protein purifications. Q Separopore 4B provides good separation over a wide molecular weight range. Larger volumes available at discounted prices (enquire).

Technical specifications:

• Matrix:Q Separopore B (crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 μm
• Activation Method: quaternary ammonium epoxy
• Molecular weight range: 6 x 10⁴ - 2 x 10⁷
• pH stability: 2 -12
• Flow specifications: 70 - 140 cm / h
• Supplied as a slurry in 20% ethanol
• Physical stability: Negligible volume variation due to changes in pH or ionic strength
• Sterilization: Chemical

References:

• Aggregation-related conformational change of the membrane-associated coat protein of bacteriophage M13. Biochemistry. (1989) 28: 9158-65.
• Quantitative assessment of comparative potencies of cholesterol-crystal-promoting factors: relation to mechanistic characterization. Biochem J. (1998) 332: 343-50.
• Fermentation Enzymes in Strictly Aerobic Bacteria: Comparative Studies on Strains of the Genus Alcaligenes and on Nocardia opaca and Xanthobacter autotrophicus. Microbiology, (1983) 129: 2825-2835.
• Immobilization of trypsin on chitosan gels: use of different activation protocols and comparison with other supports. Int J Biol Macromol. (2008) 43: 54-61.
• Quaternary ammonium substituted agarose as surface coating for capillary electrophoresis. The Analyst. (2004) pp. 410-415.