Sambucus nigra Lectin (SNA/EBL I) - Pure

Product Description
Sambucus nigra lectin (EBL I, also known as SNA I) is isolated from elderberry bark. This lectin has a molecular weight of 140,000 Da and isoelectric points between pH 5.4 and pH 5.8. It is non-specific of blood group.

EBL I belongs to the group of type 2 ribosome-inactivating proteins and is a chimeric lectin composed of an A-chain with enzymatic activity and a B-chain with carbohydrate-binding activity. It has a unique specificity of α2,6-linked sialic acid residues, and elutes 0.2M of lactose in buffered saline or acetic acid. This lectin induces caspase-dependent apoptosis at low concentrations (nM order), leading to typical symptoms of cell death in sensitive cells. This effect depends on the carbohydrate binding of the B-chain and is independent from the catalytic activity of the A-chain.

This product comes in a lyophilized powder form and is stored at -20 C. Reconstitute with sterile buffer.

Applications:
Histochemical localization of sialic acid-containing glycoconjugates
Characterization and separation of glycoprotein-derived oligosaccharides

References

• Mach, L., et al. Purification and partial characterization of a novel lectin from elder (Sambucus nigra L.) fruit. Biochem J. (1991) 278:667-671.
• Shahidi-Noghabi, S., et al. Exposure of insect midgut cells to Sambucus nigra L. agglutinins I and II causes cell death via caspase-dependent apoptosis. J Insect Physiol. (2010) 56(9):1101-7.