Sambucus nigra Lectin (SNA/EBL II) - Pure

Product Description
Sambucus nigra lectin (EBL II, also known as SNA II) is isolated from elderberry bark. This lectin is a glycoprotein containing 7.8% carbohydrate and is not blood group specific. It is composed of two subunits and has a molecular weight of 140,000 Da. EBL II has a specificity for carbohydrates containing terminal N-acetylgalactosamine and galactose residues.

The blood group A-active trisaccharide containing L-fucosyl group linked α1-2 to galactose is 10-fold lesser as an inhibitor than the parent oligosaccharide (GalNAc α1-3Gal), which suggests steric hindrance to binding by the α-L-fucosyl group and explains the failure of the lectin to exhibit blood group A specificity.

EBL II consists only of carbohydrate-binding B-chains and induces caspase-dependent apoptosis at low concentrations (nM) order, leading to typical symptoms of cell death in sensitive cells. This effect seems dependent on the carbohydrate binding B-chain.

This product comes in a lyophilized powder form and is stored at -20 C.

Applications:
Glycobiology

References

• Kaku, H., et al. Isolation and characterization of a second lectin (SNA-II) present in elderberry (Sambucus nigra L.) bark. (1990) 277(2):255-62.
• Shahidi-Noghabi, S., et al. Exposure of insect midgut cells to Sambucus nigra L. agglutinins I and II causes cell death vis caspase-dependent apoptosis. J Insect Physiol. (2010) 56(9):1101-7.