Separopore ® (Agarose) 2B-CL

Applications:

Separopore 2B-CL is a cross-linked bioagarose medium for gel filtration and coupling of affinity ligands. Hydrophilic backbone of Separopore 2B-CL results in low non-specific interactions and high protein recoveries. Separopore 2B-CL provides an excellent separation of proteins over a wide molecular weight range. Larger volumes available at discounted prices (enquire).

Technical specifications:

• Matrix: Separopore 2B-CL (crosslinked agarose beads, 2%)
• Particle size range: 52 - 165 μm
• Molecular weight range: 6 x 10⁴ - 2 x 10⁷
• pH stability: 4 - 9
• Flow specifications: 70 - 140 cm / h
• Supplied as slurry in 20% ethanol
• Chemical stability: Stable to all solutions commonly used in gel filtration including 8M Urea, 6M guanidine hydrochloride
• Physical stability: Negligible volume variation due to changes in pH or ionic strength
• Sterilization: Chemical

References:

• The photophysics of merocyanine 540. A comparative study in ethanol and in liposomes. Photochem Photobiol. (1988) 48: 187-94.
• The role of high molecular weight kininogen and prothrombin as cofactors in the binding of factor XI A3 domain to the platelet surface. J Biol Chem. (2000) 275: 25139-45.
• Factor XI binding to activated platelets is mediated by residues R(250), K(255), F(260), and Q(263) within the apple 3 domain. Biochemistry. (2000) 39: 316-23.
• Heterogeneity of membrane surface proteins in Glanzmann's thrombasthenia. Blood. (1981) 57: 174-81.
• Platelet hyperactivity and abnormal Ca2+ homeostasis in diabetes mellitus. Am J Physiol Heart Circ Physiol. (2001) 280: H1480-9.
• PAR-1-stimulated factor IXa binding to a small platelet subpopulation requires a pronounced and sustained increase of cytoplasmic calcium. Biochemistry. (2006) 45: 7289-98.
• A subpopulation of platelets responds to thrombin- or SFLLRN-stimulation with binding sites for factor IXa. J Biol Chem. (2004) 279: 19854-9.
• Identification of a binding site for glycoprotein Ibalpha in the Apple 3 domain of factor XI. J Biol Chem. (2004) 279: 45470-6.
• Fluorescence study of binding of adenosine derivatives to phospholipid membranes — effect of serum albumin. Journal of Molecular Structure (2001) 565-566: 209-212.

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• Separopore 6B-CL (SKU # 20181036)
• Separopore 6B-MB (Macrobeads) (SKU # 20181035)

Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.