Separopore ® (Agarose) 2B-MB (Macrobeads)

Applications:

Separopore 2B (macrobeads) is an agarose bead matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Larger volumes available at discounted prices (enquire).

Technical specifications:

• Matrix: Separopore 2B (agarose macrobeads 2%)
• Particle size range: 165 - 275 μm
• Molecular weight range: 6 x 10⁴ - 2 x 10⁷
• Flow specifications: 70 - 140 cm / h
• pH stability: 4 - 9
• Supplied as slurry in 20% ethanol
• Chemical stability: Stable to all solutions commonly used in gel filtration including 8M Urea, 6M guanidine hydrochloride
• Physical stability: Negligible volume variation due to changes in pH or ionic strength
• Sterilization: Chemical

References:

• The photophysics of merocyanine 540. A comparative study in ethanol and in liposomes. Photochem Photobiol. (1988) 48: 187-94.
• The role of high molecular weight kininogen and prothrombin as cofactors in the binding of factor XI A3 domain to the platelet surface. J Biol Chem. (2000) 275: 25139-45.
• Factor XI binding to activated platelets is mediated by residues R(250), K(255), F(260), and Q(263) within the apple 3 domain. Biochemistry. (2000) 39: 316-23.
• Heterogeneity of membrane surface proteins in Glanzmann's thrombasthenia. Blood. (1981) 57: 174-81.
• Platelet hyperactivity and abnormal Ca2+ homeostasis in diabetes mellitus. Am J Physiol Heart Circ Physiol. (2001) 280: H1480-9.
• PAR-1-stimulated factor IXa binding to a small platelet subpopulation requires a pronounced and sustained increase of cytoplasmic calcium. Biochemistry. (2006) 45: 7289-98.
• A subpopulation of platelets responds to thrombin- or SFLLRN-stimulation with binding sites for factor IXa. J Biol Chem. (2004) 279: 19854-9.
• Identification of a binding site for glycoprotein Ibalpha in the Apple 3 domain of factor XI. J Biol Chem. (2004) 279: 45470-6.
• Fluorescence study of binding of adenosine derivatives to phospholipid membranes — effect of serum albumin. Journal of Molecular Structure (2001) 565-566: 209-212.

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• Separopore 4B-MB (Macrobeads) (SKU #20181033)
• Separopore 6B (SKU # 20181043)
• Separopore 6B-CL (SKU # 20181036)
• Separopore 6B-MB (Macrobeads) (SKU # 20181035)

Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.