Separopore® (Agarose) 4B-CL

Applications:

Separopore 4B-CL is crosslinked high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Separopore 4B-CL is a proven base matrix for coupling affinity ligands. Separopore 4B-CL provides good separation over a wide molecular weight range. Larger volumes available at discounted prices (enquire).

Technical specifications:

• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 μm
• Molecular weight range: 6 x 10⁴ - 2 x 10⁷
• pH stability: 3 - 12
• Flow specifications: 70 - 140 cm / h
• Supplied as a slurry in 20% ethanol
• Chemical stability: Stable to all solutions commonly used in gel filtration including 8M Urea, 6M guanidine hydrochloride
• Physical stability: Negligible volume variation due to changes in pH or ionic strength
• Sterilization: Chemical

References:

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• Two monoclonal antibodies recognizing different epitopes on rat cytochrome IIB1 react with human IIE1. Mol Pharmacol. (1992) 41: 76-82.
• Isolation of a five-polypeptide cytochrome b-f complex from spinach chloroplasts. J Biol Chem. (1983) 258: 10348-54.
• The 110,000-dalton actin- and calmodulin-binding protein from intestinal brush border is a myosin-like ATPase. J Biol Chem. (1984) 259: 14128-35.
• Biotin-binding protein from egg yolk. A protein distinct from egg white avidin. J Biol Chem. (1978) 253: 6979-82.
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• Fibronectin in human gallbladder bile: cholesterol pronucleating and/or mucin "link" protein? Am J Physiol. (1994) 267: G393-400.
• Monoclonal and polyclonal antibodies recognizing acetaldehyde-protein adducts. Biochem Pharmacol. (1998) 56: 1515-23.

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Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.