Separopore ® (Agarose) 4B (Lyophilized)

Applications:

Separopore 4B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Separopore 4B is a proven base matrix for coupling affinity ligands. Separopore 4B provides good separation over a wide molecular weight range. Larger volumes available at discounted prices (enquire).

Technical Specifications:

• Matrix: Separopore 4B (agarose beads, 4%)
• Particle size range: 52 - 165 μm
• Molecular weight range: 6 x 10⁴ - 2 x 10⁷
• pH stability: 4 - 9
• Flow specifications: 70 - 140 cm / h
• Supplied Lyophilized powder, 5 g powder swells to 20 ml of gel
• Chemical stability: Stable to all solutions commonly used in gel filtration including 8M Urea, 6M guanidine hydrochloride
• Physical stability: Negligible volume variation due to changes in pH or ionic strength
• Sterilization: Chemical

References:

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• Partial purification and properties of a rat brain phospholipase. J Biol Chem. (1979) 254: 9761-5.
• Development of a novel immunoradiometric assay exclusively for biologically active whole parathyroid hormone 1-84: implications for improvement of accurate assessment of parathyroid function. J Bone Miner Res. (2001) 16: 605-14.
• Purification of the inducible murine macrophage nitric oxide synthase. Identification as a flavoprotein. J Biol Chem. (1991) 266: 22789-91.
• Cloning and characterization of novel ficolins from the solitary ascidian, Halocynthia roretzi.J Biol Chem. (2001) 276: 19959-65.
• Improvements on the purification of mannan-binding lectin and demonstration of its Ca(2+)-independent association with a C1s-like serine protease. Biochem J. (1996) 319: 329-32.
• Human plasma protein C: isolation, characterization, and mechanism of activation by alpha-thrombin. J Clin Invest. (1979) 64: 761-9.
• Induction of nerve growth factor receptor in Schwann cells after axotomy. Proc Natl Acad Sci U S A. (1986) 83: 4094-8.
• Purification and characterization of a calmodulin-dependent protein kinase that is highly concentrated in brain. J Biol Chem. (1983) 258: 12735-44.
• The role of phospholipid and factor VIIIa in the activation of bovine factor X. J Biol Chem. (1981) 256: 3433-42.
• Large, detergent-resistant complexes containing murine antigens Thy-1 and Ly-6 and protein tyrosine kinase p56lck. Eur J Immunol. (1993) 23: 825-31.
• Human adenosine deaminase. Distribution and properties. J Biol Chem. (1976) 251: 5448-56.
• Distinct forebrain and cerebellar isozymes of type II Ca2+/calmodulin-dependent protein kinase associate differently with the postsynaptic density fraction. J Biol Chem. (1985) 260: 9039-46.
• The interaction of wheat germ agglutinin with sialoglycoproteins. The role of sialic acid. J Biol Chem. (1979) 254: 4000-8.
• A membrane-bound ATPase from Halobacterium halobium: purification and characterization. J Biochem. (1987) 102: 591-8.
• Differential subcompartmentation of terminal glycosylation in the Golgi apparatus of intestinal absorptive and goblet cells. J Biol Chem. (1986) 261: 14307-12.
• Arachidonate 5-lipoxygenase of guinea pig peritoneal polymorphonuclear leukocytes. Activation by adenosine 5'-triphosphate. J Biol Chem. (1983) 258: 5754-8.
• Insulin-sepharose and the dynamics of insulin action. Proc Natl Acad Sci U S A. (1971) 68: 2066-8.
• The mechanism of thrombin-induced platelet factor 4 secretion. Blood. (1980) 55: 661-8.

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Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.