Separopore® (Agarose) 6B-CL

Applications:

Separopore 6B-CL is crosslinked high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Separopore 6B-CL is a proven base matrix for coupling affinity ligands. Separopore 6B-CL provides good separation over a wide molecular weight range. Larger volumes available at discounted prices (enquire).

Technical specifications:

• Matrix: Separopore 6B-CL (crosslinked agarose beads, 6%)
• Particle size range: 52 - 165 μm
• Molecular weight range: 6 x 10⁴ - 2 x 10⁷
• pH stability: 4 - 9
• Flow specifications: 70 - 140 cm / h
• Supplied as a slurry in 20% ethanol
• Chemical stability: Stable to all solutions commonly used in gel filtration including 8 M Urea, 6 M guanidine hydrochloride
• Physical stability: Negligible volume variation due to changes in pH or ionic strength
• Sterilization: Chemical

References:

• Aggregation-related conformational change of the membrane-associated coat protein of bacteriophage M13. Biochemistry. (1989) 28: 9158-65.
• Quantitative assessment of comparative potencies of cholesterol-crystal-promoting factors: relation to mechanistic characterization. Biochem J. (1998) 332: 343-50.
• Fermentation Enzymes in Strictly Aerobic Bacteria: Comparative Studies on Strains of the Genus Alcaligenes and on Nocardia opaca and Xanthobacter autotrophicus. Microbiology, (1983) 129: 2825-2835.
• Immobilization of trypsin on chitosan gels: use of different activation protocols and comparison with other supports. Int J Biol Macromol. (2008) 43: 54-61.
• Elevated serum levels in human pregnancy of a molecule immunochemically similar to eosinophil granule major basic protein. J Exp Med. (1983) 158: 1211-26.
• Penicillin G acylase catalyzed acylation of 7-ACA in aqueous two-phase systems using kinetically and thermodynamically controlled strategies: improved enzymatic synthesis of 7-[(1-hydroxy-1-phenyl)-acetamido]-3-acetoxymethyl-Δ3-cephem-4-carboxylic acid. Enzyme and Microbial Technology, (2005) 36: 672-679.
• Immobilization of the acylase from Escherichia coli on glyoxyl-agarose gives efficient catalyst for the synthesis of cephalosporins. Enzyme and Microbial Technology,(2008) 42: 121-129.

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• Separopore 4B (SKU # 20181031)
• Separopore 4B-CL (SKU # 20181032)
• Separopore 4B-MB (Macrobeads) (SKU #20181033)
• Separopore 6B (SKU # 20181043)
• Separopore 6B-MB (Macrobeads) (SKU # 20181035)

Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.