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50 µg

Ref. SBB-PS0028-C050
SOUTH BAY BIO

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Neuf 302.00 H.T.
en stock

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Détails Produit

Product Description: SUMO1 is a ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. This SUMO1 substrate is C-terminally derivatized with a bis-Gly-Rhodamine 110 fluorophore. The bis-Gly-Rh110 is quenched until the amide bond between the C-terminal glycine and the bis-Gly-Rh110 compound is hydrolyzed to mono-Gly-Rhodamine 110. The efficiency of quenching combined with the powerful signal upon hydrolysis yields a reagent with unparalleled signal-to-background. SUMO1-Rh110 can be used to study the deSUMOylating activity of hydrolases SENP1 and SENP2, or other deSUMOylating enzymes. The substrate activity of SUMO1-Rhodamine 110 was determined by measuring the SENP1 catalyzed release of unquenched mono-Gly-Rh110.

Alternate Names/Synonyms: Small Ubiquitin-related Modifier 1; GAP-modifying Protein 1; SMT3C, SMT3H3, UBL1; Sentrin; Ubiquitin-homology Domain Protein PIC1

Product type: Protein

Source / Host: E. coli

Sequence: Human SUMO1 (aa1-97) conjugated at the C-terminus to a quenched Rhodamine 110 dye.

Species Crossreactivity: Human

Molecular Weight: ~12kDa

Labels - Conjugates: Rhodamine

Purity: >97% (LCMS)

Concentration: Lot dependent.

Formulation: Liquid. In 50mM HEPES pH 7.5, 100mM sodium chloride.

Handling Advice: Aliquot to avoid freeze/thaw cycles. Protect from light.

Short Term Storage: -80°C

Long Term Storage: -80°C

Shipping: DRY ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -80°C.

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